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Received:February 22, 2022 Published Online:November 20, 2022
Received:February 22, 2022 Published Online:November 20, 2022
中文摘要: 目的 探讨血清miR-212、miR-21、miR-193b、miR-200c和miR-181d对胰腺癌的诊断价值。方法收集2018年7月至2021年8月南通大学附属医院43例胰腺炎患者（胰腺炎组），76例胰腺癌患者（胰腺癌组）及59例正常人（正常组）的血清，利用自建基因芯片法检测研究对象血清miR-212、miR-21、miR-193b、miR-200c和miR-181d水平，受试者工作特征（ROC）曲线分析5种miRNAs单独及联合诊断胰腺癌的效能。结果 相较于正常组及胰腺炎组，胰腺癌组5种miRNA水平明显升高，差异有统计学意义（P<0.05）。ROC曲线显示，5种miRNA均有较高的ROC曲线下面积（AUC），根据各自最佳临界值，miR-212、miR-21、miR-193b、miR-200c和miR-181d对胰腺癌诊断的敏感度分别为68.40%、67.11%、61.80%、69.70%和68.40%，特异度分别为86.30%、85.29%、74.50%、87.30%和88.20%，其中miR-200c敏感度最高，miR-181d特异度最高。两两联合检测结果显示，灵敏度最高的是miR-21/miR-200c和miR-21/miR-181d（92.11%），特异度最高的是miR-200c/miR-181d（79.41%）。结论 利用自建基因芯片检测提示，miR-212、miR-21、miR-193b、miR-200c和miR-181d在胰腺癌患者血清中水平升高，其可作为胰腺癌诊断的血清标记物，联合检测可提高胰腺癌的诊断灵敏度。
Abstract:Objective To investigate the diagnostic value of serum miR-212, miR-21, miR-193b, miR-200c and miR-181d in patients with pancreatic cancer. Methods The serum samples were collected from 59 normal people（normal group）, 43 patients with pancreatitis（pancreatitis group） and 76 patients with pancreatic cancer（pancreatic cancer group） in the Affiliated Hospital of Nantong University between July 2018 and August 2021. miR-212, miR-21, miR-193b, miR-200c and miR-181d were detected by built-in self-testing of gene chip. Receiver operating characteristic (ROC) curve was used to analyze the efficacy of five miRNAs alone and combination in the diagnosis of pancreatic cancer. Results Compared with those in normal group and pancreatitis group, the levels of five miRNAs significantly increased in pancreatic cancer group (P<0.05). ROC curve showed that all the five miRNAs had a higher area under curve. According to their cut-off point, the sensitivity of miR-212, miR-21, miR-193b, miR-200c and miR-181d in diagnosis of pancreatic cancer was 68.40%, 67.11%, 61.80%, 69.70% and 68.40%, respectively, and the specificity was 86.30%, 85.29%, 74.50%, 87.30% and 88.20%, respectively. miR-200c had the highest sensitivity, and miR-181d had the highest specificity to predict pancreatic cancer. The pairwise combinations of five miRNAs showed that miR-21/miR-200c and miR-21/miR-181d had the highest sensitivity (92.11%), and miR-200c/miR-181d had the highest specificity (79.41%).Conclusions In patients with pancreatic cancer, the serum levels of miR-212, miR-21, miR-193b, miR-200c and miR-181d are elevated by gene chip assay, which can be used as serum tumor markers for the diagnosis of pancreatic cancer. Combined detection of them can increase the diagnostic sensitivity of pancreatic cancer.
keywords: Pancreatic cancer Gene chip Micro ribonucleic acids miR-212 miR-21 miR-193b miR-200c miR-181d
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基金项目:国家自然科学基金（82272624）；江苏省自然科学基金（BK20211105）；江苏省卫健委面上项目（H2019072）；南通市科技计划项目（MS22022044，JC22022001，JCZ2022027，JC2021091）；南通市卫健委面上项目（MS2022056）
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