Abstract:Objective To explore the effects of overexpression of integrin β6 on gene expression of human colon cancer SW480 cells to reveal a potential mechanism of integrin β6 in promoting invasion and metastasis of human colon cancer cells. Methods SW480 cell line stably overexpressing integrin β6 (SW480/β6-OE) and SW480 cells transfected with empty vector (SW480/Mock) were established by lentivirus vector. The overexpression efficacy of integrin β6 was detected by western blot, and the effect of overexpression integrin β6 on the invasion ability of SW480 cells was detected by transwell assay. High throughput transcriptome sequencing technology was used to analyze the changes in gene expression profile of SW480 cells after overexpression of integrin β6, and the GO and KEGG function enrichment analysis was conducted for differentially expressed genes（DEGs）. Results Overexpression of integrin β6 significantly enhanced the invasion ability of SW480 cells(P<0.05). Compared with SW480/Mock cells, 1472 DEGs were screened from SW480/β6-OE cells, including 840 up-regulated genes and 632 down-regulated genes. KEGG enrichment analysis revealed that DEGs were enriched in the pathways involved in cancer, mitogen-activated protein kinase (MAPK), PI3K-AKT, cytokine-cytokine receptor interaction, cAMP signal pathway and Wnt signal pathway. Conclusions Overexpression of integrin β6 promotes the invasion and metastasis of SW480 cells and changes the gene expression profile of colon cancer cells. The multiple signaling pathways in which DEGs are significantly enriched have been confirmed to play an important role in the occurrence and development of colon cancer.