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Received:April 25, 2022 Published Online:November 20, 2022
Received:April 25, 2022 Published Online:November 20, 2022
中文摘要: 目的 研究结直肠癌细胞HCT116中长链非编码RNA LINC00467的相对表达水平,探索LINC00467对HCT116细胞增殖和血管生成的影响及可能的分子机制。方法使用生物信息学方法分析LINC00467在结直肠癌中的相对表达水平和核质分布情况,通过qRT-PCR检测结直肠癌细胞HCT116及人正常结肠上皮细胞株FHC中LINC00467的相对表达水平。克隆形成和基质胶成管实验检测LINC00467在HCT116中的部分生物学功能。生物信息学和双荧光素酶报告基因实验探索LINC00467和miR-128-3p的相互作用。结果 生物信息学分析显示LINC00467在结直肠癌组织中表达上调,LINC00467在HCT116细胞中表达量较FHC升高(P<0.05)。敲低LINC00467后HCT116细胞增殖和血管生成能力降低(P<0.01)。核质分离实验显示LINC00467主要位于HCT116细胞的胞质。生物信息学预测LINC00467与miR-128-3p相结合,双荧光素酶报告基因实验显示LINC00467竞争性吸附miR-128-3p。拯救实验显示LINC00467靶向miR-128-3p促进HCT116细胞增殖和血管生成。结论 LINC00467可能通过竞争性吸附miR-128-3p促进结直肠癌细胞HCT116增殖和血管生成。
Abstract:Objective To study the expression level of long-chain non-coding RNA LINC00467 in colorectal cancer cells HCT116, and to explore the effect of LINC00467 on the proliferation and angiogenesis of HCT116 cells and its possible molecular mechanism. MethodsBioinformatics method was used to analyze the relative expression level and nucleocytoplasmic distribution of LINC00467 in colorectal cancer. The relative expression levels of LINC00467 in HCT116 cells and human normal colon epithelial cell line FHC were detected by qRT-PCR. Some of the biological function of LINC00467 in HCT116 cells was detected by colony formation and matrigel tube formation assays. The interaction between LINC00467 and miR-128-3p was studied by dual-luciferase reporter assay and bioinformatics analysis. ResultsBioinformatics analysis showed that LINC00467 was up-regulated in colorectal cancer tissues, and its expression level in HCT116 cells was significantly higher than that in FHC cells (P<0.05). After knocking down LINC0067, the proliferation and angiogenesis ability of HCT116 cells decreased (P<0.01). Nucleocytoplasmic separation experiments demonstrated that LINC00467 was mainly located in the cytoplasm of HCT116 cells. Bioinformatics predicted that LINC00467 could interact with miR-128-3p, and dual-luciferase reporter gene experiments showed that LINC00467 competitively bounded to miR-128-3p. Rescue experiments showed that LINC00467 targeted miR-128-3p to promote HCT116 cell proliferation and angiogenesis.ConclusionsLINC00467 may promote the proliferation and angiogenesis of HCT116 colorectal cancer cells through competitive adsorption of miR-128-3p.
keywords: Colorectal cancer Long-chain non-coding RNA LINC00467 miR-128-3p Proliferation Angiogenesis
文章编号: 中图分类号: 文献标志码:A
基金项目:江苏省高层次卫生人才“六个一工程”拔尖人才项目(LGY2019078);“789”卓越人才培养计划(789ZYRC202090147)
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