Abstract:Objective To study the expression level of long-chain non-coding RNA LINC00467 in colorectal cancer cells HCT116, and to explore the effect of LINC00467 on the proliferation and angiogenesis of HCT116 cells and its possible molecular mechanism. MethodsBioinformatics method was used to analyze the relative expression level and nucleocytoplasmic distribution of LINC00467 in colorectal cancer. The relative expression levels of LINC00467 in HCT116 cells and human normal colon epithelial cell line FHC were detected by qRT-PCR. Some of the biological function of LINC00467 in HCT116 cells was detected by colony formation and matrigel tube formation assays. The interaction between LINC00467 and miR-128-3p was studied by dual-luciferase reporter assay and bioinformatics analysis. ResultsBioinformatics analysis showed that LINC00467 was up-regulated in colorectal cancer tissues, and its expression level in HCT116 cells was significantly higher than that in FHC cells (P<0.05). After knocking down LINC0067, the proliferation and angiogenesis ability of HCT116 cells decreased (P<0.01). Nucleocytoplasmic separation experiments demonstrated that LINC00467 was mainly located in the cytoplasm of HCT116 cells. Bioinformatics predicted that LINC00467 could interact with miR-128-3p, and dual-luciferase reporter gene experiments showed that LINC00467 competitively bounded to miR-128-3p. Rescue experiments showed that LINC00467 targeted miR-128-3p to promote HCT116 cell proliferation and angiogenesis.ConclusionsLINC00467 may promote the proliferation and angiogenesis of HCT116 colorectal cancer cells through competitive adsorption of miR-128-3p.