Abstract:Objective To investigate the expressions of ERG (Ets-related gene)-associated protein and alpha-methylacyl-CoA racemase (AMACR,P504s) in prostate biopsy specimens and their clinicopathological significance. Methods Out of 60 prostate biopsy specimens collected from Maanshan Central Hospital of Anhui Province,there were 50 cases confirmed as prostate cancer (observation group) and 10 cases diagnosed as normal prostate tissue (control group).The immunohistochemistry method was used to detect the expressions of ERG and P504s in biopsy tissues,and the relationship between the expression levels of ERG and P504s and the clinicopathological characteristics was analyzed. Results There was no statistical difference in positive expression rate of ERG protein between two groups,even though it was slightly higher in observation group (24.0% vs 0,χ²=1.688,P=0.194),but the positive expression rate of P504s protein in observation group was significantly higher than that in control group (96.0% vs 0,χ²=46.188,P=0.000).The correlation analysis showed that ERG expression was not correlated with age,preoperative serum prostate-specific antigen (PSA) value (all P>0.05),but was significantly correlated with Gleason grading,International Society of Urological Pathology(ISUP) grouping and number of positive punctures (P<0.01,P<0.05),and the positive expression rate of ERG in Gleason grade 3 prostate cancer was higher than that in Gleason grade 4 (64.3% vs 8.3%,χ²=14.370,P=0.000).P504s expression was not significantly associated with age,Gleason classification,serum PSA value,ISUP grouping,and number of positive punctures(all P>0.05). Conclusions ERG and P504s are not expressed in normal prostate tissue,but expressed and over expressed in prostate cancer,respectively.ERG expression is mostly in Gleason 3 grade of prostate cancer.Both indexes have certain value in the diagnosis of prostate cancer.