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Received:October 15, 2019 Published Online:July 20, 2020
Received:October 15, 2019 Published Online:July 20, 2020
中文摘要: 目的 探讨干扰组织蛋白酶F(CTSF)的表达对胃癌HGC-27荷瘤裸鼠肿瘤体内增殖能力的影响。
方法 30只Balb/c裸鼠采用皮下注射胃癌HGC-27细胞建立成瘤模型,然后随机分为对照组、顺铂组与联合组,每组10只。顺铂组给予顺铂注射治疗;联合组在与顺铂组治疗的基础上采用慢病毒感染载体CTSF-短发夹RNA(CTSF-shRNA)注射给予干扰CTSF表达治疗;对照组给予相同剂量相同注射方法的生理盐水治疗,三组均治疗观察4周。记录三组裸鼠的肿瘤体积、血管内皮生长因子(VEGF)与细胞凋亡变化情况。
结果 荷瘤裸鼠治疗第2、4周的肿瘤体积、CTSF与VEGF mRNA及蛋白相对表达水平均依对照组、顺铂组、联合组之序递降,组间两两比较均有统计学差异(P<0.01,P<0.05)。治疗第2、4周的肿瘤组织细胞凋亡指数依对照组、顺铂组、联合组之序递升,组间两两比较均有统计学差异(P<0.01,P<0.05)。
结论 干扰CTSF的表达联合顺铂治疗较单纯顺铂治疗对抑制胃癌HGC-27荷瘤裸鼠肿瘤体内增殖能力、促进肿瘤组织细胞凋亡、抑制VEGF表达的作用更明显,从而具有很好的抑瘤效果。
Abstract:Objective To investigate the effect of interfering cathepsin F (CTSF) expression on the proliferation of gastric carcinoma HGC-27 cells in tumor-bearing nude mice.
Methods Thirty Balb/c nude mice were injected subcutaneously with HGC-27 cells to establish tumor-forming models,which were randomly divided into control group,cisplatin group and combination group (n=10,each).Cisplatin injection was given in cisplatin group;CTSF short hairpin RNA (CTSF-shRNA)lentivirus vector was used to interfere with CTSF expression in combination group based on the same cisplatin treatment;the same dose of normal saline was given in control group.Treatment regime was continued for 4 weeks in three groups.The tumor volume,vascular endothelial growth factor (VEGF) and apoptosis were observed and recorded in three groups.
Results At 2nd and 4th week after treatment,there were significant differences in tumor volume,relative expression levels of CTSF,VEGF mRNA and protein by pairwise comparison,which statistically decreased in the order of control group,cisplatin group and combination group (P<0.01,P<0.05),and the apoptosis index in tumor tissues increased statistically in the order of control group,cisplatin group and combination group (P<0.01,P<0.05).
Conclusion Compared with cisplatin alone,interfering CTSF expression combined with cisplatin have more advantages in inhibiting the proliferation of HGC-27 cells and VEGF expression and promoting apoptosis of tumor cell in tumor bearing nude mice with better anti-tumor effect.
keywords: Cathepsin F Gastric carcinoma Tumor-bearing nude mice Apoptosis Vascular endothelial growth factor Tumor proliferation
文章编号: 中图分类号: 文献标志码:B
基金项目:沈阳市科技计划项目(18-014-4-22)
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