Abstract:Objective To explore the expression of sirtuin 6 (Sirt6) and lipid metabolism indicators in type 2 diabetic nephropathy (T2DN), and analyze their correlation with renal function indicators and their effectiveness in predicting T2DN. Methods The data of 160 patients with type 2 diabetes admitted to Langfang People's Hospital from January 2019 to November 2020 were retrospectively analyzed. The patients were divided into the observation group (n=80, T2DN) and the control group (n=80, merely type 2 diabetes) according to whether they had nephropathy or not. Sirt6, lipid metabolism indicators ［triglyceride (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C)］ , and renal function indicators ［blood urea nitrogen (BUN) and blood creatinine (Cr)］ were measured by qRT-PCR, enzyme-linked immunosorbent assay and fully automated biochemical analyzer, respectively. The serum levels of Sirt6, lipid metabolism indicators and renal function indicators between the two groups were compared, the correlation between Sirt6, lipid metabolism indicators, and renal function indicators was analyzed by Pearson correlation. Receiver operating characteristic (ROC) curve was used to analyze the efficacy of Sirt6, lipid metabolism indicators in the diagnosis of T2DN. Results The relative expression level of Sirt6 mRNA in the observation group was lower than that in the control group, and the levels of TG, TC, LDL-C, BUN and Cr were higher than those in the control group (P＜0.05). Pearson correlation analysis showed that Sirt6 was negatively correlated with BUN (r=-0802，P＜0.05) and Cr (r=-0.785，P＜0.05); TG was positively correlated with BUN(r=0.621，P＜0.05) and Cr (r=0.628，P＜0.05); TC was positively correlated with BUN (r=0.768, P＜0.05) and Cr (r=0.759, P＜0.05); LDL-C was positively correlated with BUN (r=0.722, P＜0.05) and Cr (r=0.718, P＜0.05). The area under the ROC curve (AUC) of Sirt6, TG, TC and LDL-C for diagnosis of T2DN was 0.783, 0.588, 0.605 and 0.664, respectively, while the AUC (0.875), sensitivity (92.50%), and specificity (93.75%) of combination of the four indexes were the highest. Conclusion Sirt6 is low expressed in T2DN and negatively correlated with renal function indicators. The lipid metabolism indicators are highly expressed in T2DN and positively correlated with renal function indicators. If the Sirt6 value decreases and the levels of lipid metabolism indicators increase in patients with type 2 diabetes, it reflects that the risk of renal impairment has an increscsing trend, which might lead to renal dysfunction.