Abstract:Objective To investigate the effect of nervous presynaptic membrane protein（Munc-18）antibody (anti- Munc-18) on hippocampal neurons in SD rats to explore the role of anti-Munc-18 in the pathogenesis of epileptic encephalopathy. Methods Ten SD rats were selected to construct the epilepsy mode. After successful modeling, hippocampus cortex tissues of rats were taken. The hippocampal cells were separated and cultured by trypsin digestion and were divided into anti-Munc-18 interference group and blank control group. Munc-18 antibodies of different concentrations (diluted ×200, ×400, ×1 000)and the same volume of culture medium were added for acting time of20 h or 40 h in anti-Munc-18 interference group, and the same volume of culture medium was added only in blank control group. To study thedamage of anti-Munc-18 to hippocampal neurons, enzyme-labeled instrument was used to detect cell survival rate; lactate dehydrogenase (LDH) assay kit was used to detect LDH release quantity; TdT mediated dUTP nick end labeling (TUNEL) was used to detect cell apoptosis and calculate apoptotic index (AI). Results There were significant differences in LDH release quantity, AI and cell survival rate for different concentrations of Munc-18 antibodies inanti-Munc-18 interference group （all P<0.01）. Compared with blank control group, LDH release quantity and AI increased, and cell survival rate decreased at 20 h and 40 h of acting in anti-Munc-18 interference group（all P＜0.05). Conclusion Munc-18 antibody inducing- neuronal apoptosis and damaging neurons may be an important cause of epileptic encephalopathy.