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投稿时间:2023-03-21 网络发布日期:2023-12-20
投稿时间:2023-03-21 网络发布日期:2023-12-20
中文摘要: 目的 探讨皮肤再生医疗技术湿润暴露疗法(MEBT)/湿润烧伤膏(MEBO)对糖尿病创面诱导型一氧化氮合酶(iNOS)、精氨酸酶1(Arg1)表达水平的影响。
方法选取48只Wistar雄性大鼠随机分为4组,空白组、MEBO组、贝复新组和对照组,每组各12只,其中空白组构建正常大鼠急性创面模型,MEBO组、贝复新组和对照组构建糖尿病大鼠创面模型。模型建立后,MEBO组和贝复新组分别给予创面外敷两层MEBO纱条和重组牛碱性成纤维细胞生长因子凝胶(贝复新?)纱布,对照组和空白组以两层生理盐水纱布覆盖。通过HE染色、免疫荧光法和qRT-PCR检测观察各组大鼠造模后第3、7、14天创面愈合情况、组织形态学变化,以及创面组织中M1/M2型巨噬细胞标志物iNOS、Arg1 mRNA表达变化。
结果 (1) 造模后第7、14天,MEBO组、空白组、贝复新组愈合率均高于对照组(P<0.05);(2) 造模第3天,MEBO组、对照组和贝复新组iNOS mRNA表达水平高于空白组,而Arg1 mRNA表达水平低于空白组;造模后第7、14天,与对照组相比,MEBO组、贝复新组Arg1 mRNA表达水平较高,iNOS mRNA表达水平较低(P<0.05)。
结论 MEBT/MEBO可加速糖尿病创面愈合,其作用机制可能与抑制iNOS、促进Arg1表达,参与调控巨噬细胞M1/M2极化有关。
Abstract:Objective To investigate the effect of moist exposed burn therapy (MEBT) /moist exposed burn ointment (MEBO) on the expression of inducible nitric oxide synthase (iNOS) and arginase 1 (Arg1) in diabetic wounds.
Methods Forty-eight male Wistar rats were randomly divided into four groups: blank group, MEBO group, Beifuxin group and control group, with 12 rats in each group. The blank group was used to build the acute wound model of normal rats, and the MEBO group, Beifuxin group and control group were used to build the wound model of diabetes rats. After the establishment of the model, MEBO group and Beifuxin group were given two layers of MEBO gauze and recombinant bovine basic fibroblast growth factor gel (Beifuxin?) gauze for external application on the wound surface respectively, and the control group and the blank group were covered with two layers of normal saline gauze. HE staining, immunofluorescence and qRT-PCR were used to observe the wound healing, histomorphological changes and the expression changes of M1/M2 macrophage markers iNOS and Arg1 in the wound tissue of rats in each group on the 3rd, 7th and 14th days after modeling.
Results On the 7th and 14th days after modeling, the healing rates of MEBO group, blank group and Beifuxin group were higher than those of the control group (P<0.05). On the third day of modeling, the expression level of iNOS mRNA in MEBO group, control group and Beifuxin group was higher than that in blank group, while the expression level of Arg1 mRNA was lower than that in blank group (P<0.05). On the 7th and 14th day after modeling, compared with the control group, the expression level of Arg1 mRNA in MEBO group, and Beifuxin group was higher, while the expression level of iNOS mRNA was lower (P<0.05).
Conclusion MEBT/MEBO can accelerate diabetic wound healing, and its mechanism may be related to inhibiting iNOS, promoting Arg1 expression, and participating in regulating macrophage M1/M2 polarization.
文章编号: 中图分类号:R587.2 R632.1 文献标志码:A
基金项目:国家自然科学基金(81774327);广西医学高层次骨干人才“139”计划培养人选(桂卫科教发〔2018〕22号);右江民族医学院研究生创新计划项目(YXCXJH2022004)
引用文本:
杨雅量,葛星月,李文武,等.皮肤再生医疗技术对糖尿病创面诱导型一氧化氮合酶和精氨酸酶1表达水平的影响[J].中国临床研究,2023,36(12):1836-1841.
杨雅量,葛星月,李文武,等.皮肤再生医疗技术对糖尿病创面诱导型一氧化氮合酶和精氨酸酶1表达水平的影响[J].中国临床研究,2023,36(12):1836-1841.