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投稿时间：2023-05-24 网络发布日期：2023-12-20
投稿时间：2023-05-24 网络发布日期：2023-12-20
中文摘要: 目的 研究小檗碱基于磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(Akt)通路对2型糖尿病（T2DM）大鼠炎症水平和糖脂代谢的影响。
方法选取40只SPF级SD雄性大鼠，其中随机选取10只实验大鼠为正常组，其余30只建造T2DM模型［随机分为模型组（10只）、二甲双胍阳性对照组（10只）、小檗碱组（10只）］。检测大鼠血清炎症因子［肿瘤坏死因子-α（TNF-α）、 C-反应蛋白（CRP）、白细胞介素-1β（IL-1β）］，脂代谢指标［低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、三酰甘油（TG）、总胆固醇（TC）、游离脂肪酸（FFA）］和糖代谢指标［空腹胰岛素（FINS）、空腹血糖（FBG）、胰岛素抵抗指数（HOMA-IR）］，检测胰腺组织中PI3K、Akt和p-PI3Kp85蛋白的表达。
结果 T2DM模型三个组的血清糖代谢指标（FBG、FINS、HOMA-IR）、脂代谢指标（TC、TG、LDL-C、FFA）、炎症因子（TNF-α、CRP、IL-1β）水平均高于正常组（P＜0.05）；T2DM模型三个组中，依模型组、二甲双胍阳性对照组、小檗碱组之序, 除HDL-C水平递升，其他指标水平均递降（P＜0.05）。T2DM模型三个组胰腺组织中的PI3K、Akt和p-PI3Kp85蛋白相对表达水平均低于正常组（P＜0.05）,且三者表达水平依模型组、二甲双胍阳性对照组、小檗碱组之序递升（P＜0.05）。
结论 小檗碱能改善T2DM大鼠脂代谢紊乱状况，可抑制其体内炎症反应，作用机制可能与调控PI3K/Akt通路有关。
Abstract:Objective To study the effects of berberine on inflammatory factor levels and glucose and lipid metabolism in type 2 diabetes mellitus (T2DM) rats based on phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) pathway.
Methods Forty SPF grade SD male rats were selected, of which 10 rats were randomly selected as normal group, and the other 30 rats were constructed as T2DM model rats ［divided into model group (10 rats), metformin positive control group (10 rats) and berberine group (10 rats) randomly］. The levels of inflammatory factors ［tumor necrosis factor-α (TNF-α)、 C-reactive protein (CRP), interleukin-1β (IL-1β)］, lipid metabolism indexes ［low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), total cholesterol (TC), free fatty acid (FFA)］, and glucose metabolism indexes [fasting insulin (FINS), fasting blood glucose (FBG), insulin resistance index (HOMA-IR)]. The expressions of PI3K, Akt and p-PI3Kp85 proteins in pancreatic tissue were detected. Results The levels of serum glucose metabolism indexes (FBG, FINS, HOMA-IR), lipid metabolism indexes (TC, TG, LDL-C, FFA) and inflammatory factors (TNF-α, CRP, IL-1β) in the three T2DM model groups were higher than those in the normal group (P<0.05). Among the three T2DM model groups, except for HDL-C levels that gradually increased, all other indicator levels decreased gradually in the order of model group, metformin positive control group and berberine group (P<0.05). The relative expression levels of PI3K, Akt and p-PI3Kp85 proteins in pancreatic tissues of the three T2DM model groups were lower than those of the normal group (P<0.05), while the relative expression levels of the three proteins increased sequentially in order of model group, metformin-positive control group and berberine group (P<0.05). Conclusion Berberine can improve the disorder of lipid metabolism in T2DM rats and inhibit the inflammatory reaction in vivo. The mechanism may be related to the regulation of PI3K/Akt pathway.
keywords: Berberine Metformin Type 2 diabetes mellitus Inflammation reaction Lipid metabolism Glucose metabolism Phosphatidylinositol-3-kinase/protein kinase B signal pathway
文章编号： 中图分类号：R587.1 R-33 文献标志码：A
基金项目:河北省医学科学研究课题计划（20200428）
附件
| 作者 | 单位 |
| 贾小玉 | 1. 邯郸市第二医院检验科，河北 邯郸 056001 |
| 赵婷 | 2. 邯郸市第一医院核医学科，河北 邯郸 056007 |
| 尹世伟 | 3. 邯郸市第一医院试剂科，河北 邯郸 056007 |
| 侯洋洋 | 4. 邯郸市第二医院核医学科，河北 邯郸 056001 |
| 李静 | 5. 邯郸市第二医院心内二科，河北 邯郸 056001 |
引用文本：
贾小玉,赵婷,尹世伟,等.小檗碱通过抑制PI3K/Akt对2型糖尿病大鼠炎症因子水平和糖脂代谢的影响[J].中国临床研究,2023,36(12):1831-1835.
贾小玉,赵婷,尹世伟,等.小檗碱通过抑制PI3K/Akt对2型糖尿病大鼠炎症因子水平和糖脂代谢的影响[J].中国临床研究,2023,36(12):1831-1835.