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投稿时间:2023-02-18 网络发布日期:2023-07-19
投稿时间:2023-02-18 网络发布日期:2023-07-19
中文摘要: 目的 探讨黄豆苷元对乳腺癌MCF-7和MDA-MB-231细胞增殖、迁移和侵袭等生物学行为的影响及其相关机制。
方法 体外培养乳腺癌MDA-MB-231、MCF-7细胞,应用CCK-8实验、平板克隆形成实验、细胞划痕实验以及Transwell实验,检测不同浓度黄豆苷元(0、40、80、160μmol/L)对乳腺癌细胞的活力、克隆形成能力、迁移与侵袭的影响;应用Western Blot法检测乳腺癌细胞中Wnt/β-catenin信号通路相关蛋白(Wnt-3a,β-catenin)和上皮—间质转化(EMT)相关蛋白[E-cadherin、Vimentin、Slug、Twist)的表达。
结果 黄豆苷元对乳腺癌MDA-MB-231、MCF-7细胞的活力有明显抑制作用且呈剂量依赖关系(P<0.05);随着黄豆苷元浓度增加,乳腺癌细胞的克隆形成能力逐步降低,迁移、侵袭能力逐渐减弱(P<0.05)。黄豆苷元的干预显著提高乳腺癌细胞E-cadherin表达水平,降低β-catenin、Wnt-3a、Vimentin、Slug、Twist蛋白表达水平(P<0.05)。
结论 黄豆苷元可能通过影响细胞Wnt/β-catenin信号通路相关蛋白和EMT相关蛋白的表达,抑制乳腺癌细胞MCF-7和MDA-MB-231的增殖、侵袭和迁移能力,进而抑制肿瘤的发展。
Abstract:Objective To investigate the effects of daidzein on proliferation, migration and invasion of breast cancer cell line MCF-7 and MDA-MB-231 and its related mechanisms.
Methods Breast cancer MDA-MB-231 and MCF-7 cells were cultured in vitro. The effects of daidzein at different concentrations (0,40,80,160μmol/L) on the viability, colony forming ability, migration and invasion of breast cancer cells were detected by CCK-8 test, plate clone formation test, cell scratch test and Transwell assay. The expression of Wnt/ β-catenin signaling pathway related proteins (Wnt-3a, β-catenin) and epithelial-mesenchymal transition (EMT) related proteins (E-cadherin, Vimentin, Slug, Twist) in breast cancer cells were detected by Western Blot.
Results Daidzein significantly inhibited the viability of breast cancer cells MDA-MB-231 and MCF-7 in a dose-dependent manner (P<0.05). With the increase of daidzein concentration, the clone formation ability, migration and invasion ability of breast cancer cells decreased gradually (P<0.05). The expression level of E-cadherin in breast cancer cells increased, while the protein expression levels of β-catenin, Wnt-3a, Vimentin, Slug and Twist decreased significantly by the intervention of daidzein (P<0.05).
Conclusion Daidzein may inhibit the proliferation, invasion and migration of breast cancer cells MCF-7 and MDA-MB-231 by affecting the expression of Wnt/ β-catenin signaling pathway-related proteins and EMT-related proteins, thus inhibit tumor development.
keywords: Daidzein Breast cancer Cell proliferation Cell invasion Epithelial-mesenchymal transitions Wnt/ β-catenin signaling pathway
文章编号: 中图分类号:R-331 R737.9 文献标志码:A
基金项目:国家自然科学基金(81860580)
Author Name | Affiliation |
ZHAO Yingqi, LIU Lu, YI Peipei, CHEN Wenjun, LYU Yuanming | School of Public Health, Guilin Medical University, Guilin, Guangxi 541000, China |
引用文本:
赵英琦,刘璐,易佩佩,陈文君,吕元明.黄豆苷元促进乳腺癌细胞增殖、侵袭和迁移的机制研究[J].中国临床研究,2023,36(7):971-976.
赵英琦,刘璐,易佩佩,陈文君,吕元明.黄豆苷元促进乳腺癌细胞增殖、侵袭和迁移的机制研究[J].中国临床研究,2023,36(7):971-976.