Abstract:Objective To explore the clinical value of miRNA-125b combined with miRNA-133a in the early diagnosis and prognosis evaluation of acute respiratory distress syndrome（ARDS） in the patients with sepsis. Methods A retrospective analysis was conducted in 203 sepsis patients treated at the First Hospital of Yulin between January 2019 and December 2021. The patients complicated with ARDS were selected as observation group（n=82）, the patients without ARDS were selected as sepsis control group（n=121）, and another 90 cases undergoing physical examination were served as healthy control group. The clinical data of each group were collected,and the serum miRNA-125b and miRNA-133a levels were measured to analyze the value of miRNA-125b and miRNA-133a in the early diagnosis of ARDS in patients with sepsis and the influencing factors of them. Results The relative expression levels of miRNA-125b and miRNA-133a decreased in the order of observation group, sepsis control group and control group, with significant differences in them（P＜0.01）. In diagnosing sepsis complicated with ARDS, the optimal thresheld of miRNA-125b, miRNA-133a were 2.622, 2.317, respectively, the sensitivities of miR-125b, miR-133a and the combination of them were 84.15%, 65.85% and 84.15% and specificities of 66.12%, 83.47% and 82.64%. The area under ROC curve（AUC） were 0.818, 0.783 and 0.877, respectively, and the AUC of combined detection was respectively superior to that of miRNA-125b（Z=2.821, P=0.005） and miRNA-133a（Z=3.521, P＜0.01）.Logistic regression results showed that APACHE Ⅱ score≥25（OR=5.960）, septic shock（OR=3.511） and relative expression levels of miRNA-125b≥2.622（OR=2.542） and miRNA-133a≥2.317（OR=2.781） were the risk factors for sepsis combined with ARDS（P＜0.01）. Conclusion Combined detection of serum miRNA-125b and miRNA-133a has diagnostic and prognostic value in sepsis patients with ARDS.