Abstract:Objective To investigate the effect of feline leukemia virus subgroup C receptor 1-antisense 1 （FLVCR1-AS1） on the proliferation, migration and invasion of SiHa cervical cancer cells and its possible mechanism. Methods The cancer tissues and adjacent tissues were collected from 35 patients with cervical cancer who underwent surgery in the Second Affiliated Hospital of Hainan Medical College from December 2016 to November 2018. SiHa cells were cultured in vitro and transfected respectively with negative control siRNA (si-NC group), si-FLVCR1-AS1（si-FLVCR1-AS1 group）, miR-NC（miR-NC group）,miR-877-5p mimics（miR-877-5p group）and co-transfected with si-FLVCR1-AS1 and anti-miR-NC（si-FLVCR1-AS1+anti-miR-NC group）and with si-FLVCR1-AS1 and anti-miR-877-5p（si-FLVCR1-AS1+anti-miR-877-5p group）. CCK-8 method was used to detect the cell proliferation, and transwell method was used to detect the migration and invasion of cells. The protein expressions of Ki-67, MMP-2 and MMP-9 were determined by western blot method and the expressions of FLVCR1-AS1 and miR-877-5p were detected by RT-qPCR. The regulatory relationship between FLVCR1-AS1 and miR-877-5p was confirmed by double-luciferase reporter gene assay. Results The expression level of FLVCR1-AS1 in cervical cancer tissue was significantly higher than that in adjacent tissue (3.82±0.23 vs 1.00±0.08,t=68.510, P<0.01), and the expression level of miR-877-5p was lower than that in adjacent tissue (0.32±0.03 vs 1.00±0.05,t=68.993,P<0.01). After transfection, the expression of FLVCR1-AS1 in SiHa cells was significantly down-regulated in si-FLVCR1-AS1 group compared to si-NC group (P<0.01),the expression of miR-877-5p in SiHa cells was significantly up-regulated in miR-877-5p group compared to miR-NC group (P<0.01), and their OD value, migration and invasion of SiHa cells and the protein expressions of Ki-67, MMP-2 and MMP-9 in si-FLVCR1-AS1 group and in miR-877-5p group were significantly lower than those in si-NC group and in miR-NC group, respectively (P<0.05). After co-transfection with miR-877-5p inhibitor, the OD value, migration number, invasion number and protein expression of Ki-67, MMP-2 and MMP-9 of SiHa cells in si-FLVCR1-AS1+anti-miR-877-5p group were higher than those in si-FLVCR1-AS1+anti-miR-NC group. Conclusion FLVCR1-AS1 may promote the proliferation, migration and invasion of SiHa cervical cancer cells by targeted inhibition of miR-877-5p expression.