Abstract:Objective To analyze the perinatal complications and perinatal outcomes of twin pregnancy with different Methods of conception. Methods The clinical data were collected and retrospectively analyzed in 343 pregnant women with twin pregnancy underwent prenatal examination and delivery in Beijing Haidian Maternal and Child Health Hospital from January 2018 to December 2020. According to different Methods of conception,they were divided into assisted reproduction technology group (ART group, n =150) and natural conception group (NC group, n =193).The general situation of pregnant women, perinatal complications and perinatal outcomes were compared between two groups. Results The proportion of primiparas in ART group was higher than that in NC group，and the number of pregnancies (the average gravidity) was lower than that in NC group（ P <0.05）.Compared with control group,the incidences of pregnancy-induced hypertension and severe postpartum hemorrhage increased,and the incidence of premature delivery decreased in ART group ( P <0.05). In 686 perinatal infants, the proportion of small for gestational age(SGA) infants in ART group was statistically higher than that in NC group( P ＜0.05）. Conclusion ART pregnancy can increase the risk of perinatal complications and adverse perinatal outcomes of twin-pregnant women，so management of twin pregnancy should be strengthened to reduce the occurrence of adverse perinatal outcomes.