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投稿时间：2018-11-28 网络发布日期：2019-06-21
投稿时间：2018-11-28 网络发布日期：2019-06-21
中文摘要: 目的 探讨氧化槐果碱对人肝癌SMMC-7721细胞增殖和凋亡的影响,并探讨其可能介导的信号通路。方法 将人肝癌SMMC-7721细胞分为对照组、氧化槐果碱处理组(2、4、8、16、32 mmol/L)。将梯次浓度氧化槐果碱作用于肝癌细胞后培养48 h；采用倒置显微镜观察细胞形态变化；采用CCK8法检测不同浓度氧化槐果碱对细胞增殖的影响,采用流式细胞术检测氧化槐果碱对细胞凋亡的影响,通过免疫印迹法检测氧化槐果碱对肝癌细胞B细胞淋巴瘤/白血病-2(Bcl-2),Bcl-2相关X蛋白(Bax)
及转录激活因子(STAT)3、STAT5表达水平的影响,分析氧化槐果碱对肝癌SMMC-7721细胞可能的作用。结果 氧化槐果碱呈剂量依赖性抑制肝癌SMMC-7721细胞的增殖,并且诱导细胞凋亡。并呈剂量依赖性上调促凋亡Bax的表达,下调抗凋亡蛋白Bcl-2、STAT3、STAT5的表达。结论 氧化槐果碱对人肝癌SMMC-7721细胞有抑制增殖、诱导凋亡的作用，可能是通过酪氨酸激酶(JAK)/STAT信号通路下调STAT3、STAT5蛋白激酶表达,从而上调促凋亡Bax基因表达,下调抗凋亡Bcl-2基因表达。
Abstract:ObjectiveTo investigate the effects of oxysophocarpine on the proliferation and apoptosis of human hepatocellular carcinoma SMMC-7721 cells and its possible signaling pathways. Methods Human hepatocellular carcinoma SMMC-7721 cells were divided into control group and oxysophocarpine treatment group (2,4,8,16,32 mmol/L).The effects of different concentrations of oxysophocarpine on cell proliferation were detected by CCK8 method,the effects of oxysophocarpine on cell apoptosis were detected by flow cytometry,and the apoptotic proteins Bax,Bcl-2 were detected by immunoblotting.The effects of signal transduction and transcription activator (STAT) 3 and STAT5 on the expression level were studied.The possible signaling pathway of sophocarpine oxide on SMMC-7721 cells was analyzed. Results Oxysophocarpine inhibits the proliferation of SMMC-7721 cells in a dose-dependent manner and induces apoptosis.The expression of apoptotic Bax was up-regulated in a dose-dependent manner,and the expression of anti-apoptotic proteins Bcl-2,STAT3 and STAT5 was down-regulated. Conclusion Oxysophocarpine can inhibit proliferation and induce apoptosis of human hepatocellular carcinoma SMMC-7721 cells.This may be related to the down-regulation of STAT3 and STAT5 protein kinase expression by tyrosine kinase (JAK) / STAT signaling pathway,thus up-regulation of Bax gene expression and down-regulation of Bcl-2 gene expression.
keywords: Oxysophocarpine Hepatocellular carcinoma Proliferation Apoptosis Bcl-2 Bax JAK/STAT signaling pathway
文章编号： 中图分类号：R-33 文献标志码：B
基金项目:江苏省昆山市社会发展科技项目(ks1659)
附件
| 作者 | 单位 |
| 谢俊杰1 | 1.江苏大学医学院,江苏 镇江 212000 |
| 王庆华2 | 2.江苏大学附属昆山医院消化内科,江苏 昆山 215300 |
| 夏晓华3 | 3.江苏大学附属昆山医院急诊科,江苏 昆山 215300 |
| 韩玮4 | 4.江苏大学附属昆山医院综合外科,江苏 昆山 215300 |
| 任静1 | 1.江苏大学医学院,江苏 镇江 212000 |
| 王炳芳2 | 2.江苏大学附属昆山医院消化内科,江苏 昆山 215300 |
| Author Name | Affiliation |
| XIE Jun-jie*,WANG Qing-hua,XIA Xiao-hua, | *School of Medicine,Jiangsu University,Zhenjiang,Jiangsu 212000,China |
| HAN Wei,REN Jing,WANG Bing-fang | |
引用文本：
谢俊杰,王庆华,夏晓华,等.氧化槐果碱对肝癌SMMC-7721细胞增殖与凋亡的影响[J].中国临床研究,2019,32(6):826-829.
谢俊杰,王庆华,夏晓华,等.氧化槐果碱对肝癌SMMC-7721细胞增殖与凋亡的影响[J].中国临床研究,2019,32(6):826-829.