Abstract:ObjectiveTo investigate the effect of ethylene estradiol cycloprogesterone tablets (Diane 35) combined with human chorionic gonadotropin (HCG) on the treatment of polycystic ovarian syndrome (PCOS) and its influence on the levels of serum osteocalcin (OC) and sex hormone-binding globulin (SHBG). Methods A total of 136 PCOS patients treated from February 2015 to April 2017 were randomly divided into control group and combined group (n=68,each).Oral Diane 35 was given in control group,and Diane 35 combined with HCG intramuscular injection was given in combined group.The therapeutic effects,body mass index (BMI),whist hip ratio(WHR),homeostasis model assessment of insulin resistance(HOMA-IR),and the levels of sex hormones［follicle stimulating hormone(FSH),luteotropic hormone(LH),total testosterone (TT)］,OC,SHBG before and after treatment,ovulation induction and pregnancy status (follicle maturation time,single follicle ovulation rate,ovulation rate and pregnancy rate) after treatment were compared between two groups. Results (1) The total effective rate was 95.56% in combined group and 75.56% in control group,and there was a significant difference between two groups (χ²=11.450,P<0.01).(2) After treatment,BMI and HOMA-IR were significantly lower than those before treatment in both groups (P<0.05) and were lower in combined group than those in control group (all P<0.01),After a six-cycle treatment,WHR was also significantly lower in combined group than that in control group (P<0.01).(3) After treatment,the levels of FSH,LH and TT were significantly lower than those before treatment in two groups (P<0.05) and were significantly lower in combined group than those in control group (all P<0.01).(4) After treatment,the serum OC and SHBG levels were significantly higher than those before treatment in both groups (P<0.05) and were significantly higher in combined group than those in control group (P<0.05 or P<0.01).(5) After treatment,the ovulation rate was significantly higher than that before treatment in two groups.After 3-,6-cycle treatment,the ovulation rates were 82.35%,89.71% respectively in combined group and 41.18%,51.47% respectively in control group,and the pregnancy rates were 29.41%,52.94%,respectively in combined group and 11.76%,25.00%,respectively in control group (χ²= 24.410,23.942,6.476,11.161，P<0.05 or P<0.01). Conclusion Oral Diane 35 combined with intramuscular injection of HCG has more advantages in reducing hyperandrogenism,regulating endocrine disorders and ovulation disorders,promoting the increase of serum OC and SHBG levels and increasing the ovulation rate and pregnancy rate of patients.