Abstract:ObjectiveTo investigate the effect of color Doppler flow imaging (CDFI) combined with serum carbohydrate antigen 125 (CA125) and CA724 levels on the diagnosis of ovarian cancer (OC). Methods A total of 99 patients with ovarian mass diagnosed from March 2016 to April 2018 were enrolled.All patients received CDFI examination and serum CA125 and CA724 detection.The OC and benign lesions were detected respectively by CDFI,CA125,CA724,CDFI+CA125,CDFI+CA724,CDFI+CA125+CA724 methods.The sensitivity,accuracy,specificity and misdiagnosis rate of each combination examination were analyzed,and the levels of serum CA125,CA724,pulsation index (PI) and resistance index (RI) were compared before and 1 week after operation between benign group and malignant groups. Results Surgical pathology confirmed 48 cases of OC,51 cases of benign tumors.There were 55 cases of OC detected by CDFI,50 cases by CA125,51 cases by CA724,48 cases by CDFI+CA125,48 cases by CDFI+CA724 and 34 cases by CDFI+CA125+CA724.There were no significant differences in sensitivity and accuracy among detection methods (P>0.05).Compared with other detections,the specificity of CDFI + CA125 + CA724 increased,and the misdiagnosis rate decreased significantly (P<0.05).Before operation,the levels of CA125 and CA724 in malignant group were higher than those in benign group,and RI and PI were lower than those in benign group (P<0.05).At 1 week after operation,the levels of CA125 and CA724 in malignant group were lower than those before surgery,but higher than those in benign group (P<0.05);there were no significant differences in RI and PI between benign group and malignant group (P>0.05). Conclusion CDFI combined with serum CA125 and CA724 levels can achieve disease identification and localization,which can improve the diagnostic specificity and reduce the rate of misdiagnosis.CA125 and CA724 can be applied to the evaluation of the therapeutic efficacy of OC,providing valuable reference for the formulation of clinical follow-up treatment programs.