Abstract:Objective To analyze the relationship between XRCC1 protein, miR-7, c-erbB-2 gene expression and radiotherapy resistance in non-small cell lung cancer (NSCLC) and their predictive value. Methods From January 2020 to June 2023, 100 NSCLC patients with stage ⅢB and above who were first diagnosed and treated with radiotherapy at The Affiliated Huai'an Hospital of Xuzhou Medical University were retrospectively analyzed. The patients were divided into radiotherapy-sensitive group (n=64) and radiotherapy-resistant group (n=36) according to their radiotherapy effects, and the expression levels of XRCC1 protein, miR-7 and c-erbB-2 genes were compared between the two groups. Logistic regression was used to analyze the independent influencing factors of radiotherapy resistance in NSCLC, and the predictive value of the above factors on radiotherapy resistance in NSCLC was analyzed by ROC curve.Results The percentage of patients with tumor maximum diameter ≥5 cm, XRCC1 protein expression, miR-7 and c-erbB-2 gene expression were higher in the radiotherapy-resistant group than in the radiotherapy-sensitive group (P<0.05). Tumors maximum diameter ≥5 cm (OR=3.378, 95%CI: 1.438-7.931), XRCC1 protein high expressin (OR=5.033, 95%CI:1.994-12.708), miR-7 gene high expression (OR=3.516, 95%CI: 1.994-6.198) and c-erbB-2 gene high expression (OR=4.036, 95%CI: 1.769-9.211) were independent risk factors for radiotherapy resistance in NSCLC (P<0.05). c-erbB-2 gene predicted NCSLC radiotherapy resistance with the highest AUC value of 0.963, and the others, in order of magnitude, were XRCC1 protein (0.882), miR-7 (0.814), and tumor maximal diameter (0.648). Conclusion XRCC1 protein, miR-7, and c-erbB-2 gene expression levels were associated with NSCLC radiotherapy resistance and had good predictive value for radiotherapy resistance.