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Received:May 15, 2024 Published Online:October 20, 2024
Received:May 15, 2024 Published Online:October 20, 2024
中文摘要: 目的 检测乳腺癌患者癌组织中血管内皮生长因子（VEGF）表达及微血管密度，分析其与乳腺癌临床病理特征的关系。
方法 将2021年12月至2022年12月南京医科大学第四附属医院收治的96例乳腺癌患者作为研究对象，根据病理是否确诊为三阴性乳腺癌（TNBC），分为TNBC组24例，非TNBC组72例。采用免疫组织化学（IHC）法检测乳腺癌组织中VEGF蛋白的表达；通过IHC标记内皮细胞并计数微血管数量，即微血管密度。分析两者与乳腺癌临床病理特征的关系。
结果 TNBC患者VEGF阳性表达率（66.67%）高于非TNBC患者（25.00%），差异有统计学意义（χ2=13.662， P＜0.01）；微血管密度值（66.04±10.29）高于非TNBC患者（61.07±10.36），差异有统计学意义（t=2.039， P=0.044）。TNBC组中，临床分期Ⅰ~Ⅱ期、淋巴结无转移患者的VEGF阳性表达率显著低于临床分期Ⅲ期、淋巴结有转移患者（P＜0.05）；肿瘤直径≥2 cm、临床分期Ⅲ期患者的微血管密度值大于肿瘤直径＜2 cm、临床分期Ⅰ~Ⅱ期患者，差异有统计学意义（P＜0.05）。非TNBC组中，不同临床病理特征患者的VEGF阳性表达率及微血管密度值差异均无统计学意义（P＞0.05）。
结论 在TNBC患者中，VEGF高表达、微血管密度增高，二者与TNBC的肿瘤直径、临床分期、淋巴结转移有一定相关性，对患者预后的预测有一定帮助，可为靶向VEGF通路或微血管的治疗提供新途径。
中文关键词:
Abstract:ObjectiveTo detect the expression of vascular endothelial growth factor (VEGF) and microvessel density in cancer tissues of breast cancer patients, and to analyze their relationship with clinicopathological characteristics of breast cancer.
Methods Ninety-six breast cancer patients treated at the Fourth Affiliated Hospital of Nanjing Medical University from December 2021 to December 2022 were selected as the research subjects. According to whether the pathology was confirmed as triple-negative breast cancer (TNBC), they were divided into the TNBC group (24 cases) and the non-TNBC group (72 cases). Immunohistochemistry was used to detect the expression of VEGF protein in breast cancer tissues, and microvessel density was evaluated by immunohistochemical labeling of endothelial cells followed by counting the number of microvessels. The relationship between the two and the clinicopathological characteristics of breast cancer was analyzed.
Results The positive rate of VEGF in TNBC patients(66.67%) was higher than that in non-TNBC patients (25.00%), with a significant difference (χ2=13.662, P＜0.01); the microvessel density in TNBC patients（66.04±10.29) was higher than that in non-TNBC patients (61.07±10.36), with a significant difference (t=2.039, P=0.044). In the TNBC group, the positive expression rate of VEGF in patients with clinical stage Ⅰ-Ⅱ and no lymph node metastasis was significantly lower than that in patients with clinical stage Ⅲ and lymph node metastasis (P＜0.05), and the microvascular density in patients with tumor diameter≥2 cm and clinical stage Ⅲ was significantly higher than that in patients with tumor diameter＜2 cm and clinical stage Ⅰ-Ⅱ (P＜0.05). In non-TNBC group, there was no significant difference in VEGF positive expression rate and microvessel density between patients with different clinicopathological characteristics (P＞0.05).
Conclusion In TNBC patients, the high expression of VEGF and the increase of microvessel density are related to the tumor diameter, clinical stage and lymph node metastasis of TNBC, which is helpful to predict the prognosis of patients and provide a new way to target VEGF pathway or microvessel therapy.
keywords: Breast cancer, triple-negative Immunohistochemical staining Vascular endothelial growth factor Microvessel density Clincopathological feature
文章编号： 中图分类号：R737.9 文献标志码：A
基金项目:江苏省卫生健康委科研项目（Z2021019）
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