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Received:August 07, 2023 Published Online:June 20, 2024
Received:August 07, 2023 Published Online:June 20, 2024
中文摘要: 目的 探究溃疡性结肠炎大鼠给予黄芩素后,其辅助性T细胞(Th)1/2、血清脑肠肽因子肥胖抑制素(obestatin)及5-羟色胺(5-HT)的变化。方法 选取40只SPF级SD雄性大鼠,随机分为正常组(N组)、模型组(M组)、柳氮磺吡啶组(S组)和黄芩素组(B组),每组10只。对M、S、B组采用三硝基苯磺酸法建立溃疡性结肠炎模型,N组不建立该模型。建模成功后,对S组灌胃0.3 g/kg的柳氮磺吡啶,对B组灌胃50 mg/kg的黄芩素,N组、M组同期给予同体积生理盐水灌胃,对大鼠进行疾病活动指数(DAI)评分及结肠黏膜损伤指数(CMDI)评分,HE染色法检测结肠组织病理形态,免疫印迹法检测结肠组织中Th1标志蛋白干扰素-γ(IFN-γ)、Th2标志蛋白白细胞介素-4(IL-4)的蛋白表达,ELISA法检测大鼠血清中obestatin及5-HT水平。结果 所有大鼠造模均成功。与N组相比,M组DAI评分及CMDI评分显著升高(P<0.05);与M组比较,S、B两组显著降低(P<0.05),且B组低于S组(P<0.05)。病理结果显示,与M组相比,S组、B组病理状态明显改善,杯状细胞明显增多。与N组比较,M组结肠组织中IFN-γ蛋白表达显著升高(P<0.05),IL-4蛋白表达显著降低(P<0.05),与M组比较,S组、B组结肠组织中IFN-γ蛋白表达显著降低(P<0.05),IL-4蛋白表达显著升高(P<0.05),且B组比S组变化显著(P<0.05)。与N组比较,M组大鼠血清obestatin含量显著降低(P<0.05),5-HT含量显著升高(P<0.05);与M组比较,S组、B组血清obestatin含量显著升高(P<0.05),5-HT含量显著降低(P<0.05),且B组比S组变化显著(P<0.05)。结论 黄芩素对溃疡性结肠炎大鼠具有显著疗效,可有效调控Th1/2细胞,并显著升高血清obestatin含量,降低5-HT含量。
Abstract:Objective To investigate the effects of baicalein on T helper cells (Th) 1/2, obestatin and 5-hydroxytryptamine (5-HT) in rats with ulcerative colitis. Methods Forty SPF grade male SD rats were randomly divided into normal group (group N), model group (group M), sulfasalazine group (group S), and baicalein group (group B), with 10 rats in each group. The ulcerative colitis models were established in group M, S and B by trinitrobenzenesulfonic acid. After successful modeling, 0.3 g/kg sulfasalazine was given to group S by gavage, and 50 mg/kg baicalein was given to group B by gavage. Group N and group M were given the same volume of normal saline by gavage at the same time. The disease activity index (DAI) score and colon mucosa damage index (CMDI) score of rats were evaluated, and the pathological morphology of colon tissue was detected by hematoxylin and eosin staining. The protein expression of Th1 cytokine interferon-γ (IFN-γ) and Th2 cytokine interleukin-4 (IL-4) in colon tissues was detected by western blot, and the levels of obestatin and 5-HT in rat serum were detected by ELISA. Results All rats were successfully modeled. The DAI score and CMDI score were significantly increased in group M compared to those in group N (P<0.05), and significantly decreased in groups S and group B compared to those in group M (P<0.05), with group B being lower than group S (P<0.05). Pathological diagnosis showed that compared with group M and group S, group B had significant improvement in pathological state and a significant increase in goblet cells. Compared with group N, IFN-γ protein expression in colon tissue was significantly higher, while IL-4 protein expression was significantly lower in group M (P<0.05). Compared with group M, IFN-γ protein expression in colon tissue was significantly, while the IL-4 protein expression was significantly higher in group S and group B (P<0.05), and the changes in group B were more significant than those in group S (P<0.05). Compared with group N, the content of serum obestatin was significantly higher (P<0.05), while the 5-HT content was significantly lower in group M (P<0.05). Compared with group M, the content of serum obestratin was significantly increased (P<0.05), while the 5-HT content was significantly reduced in group S and group B (P<0.05), and the group B showed a significant change compared to group S (P<0.05). Conclusion Baicalein has significant therapeutic effects on ulcerative colitis rats, which can regulate Th1/2 cells, increase the content of serum obestatin, and reduce the content of 5-HT significantly.
keywords: Baicalein Ulcerative colitis T helper cells Brain-gut peptide Obestatin 5-hydroxytryptamine Disease activity index Sulfasalazine Interferon Interleukin Colon mucosa damage index
文章编号: 中图分类号:R285 R574.62 文献标志码:A
基金项目:国家自然科学基金青年科学基金项目(82104792);北京市平谷区卫生健康委科研项目(pgwjw2020-06)
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