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Received:March 22, 2022 Published Online:January 20, 2023
Received:March 22, 2022 Published Online:January 20, 2023
中文摘要: 目的基于网络药理学探讨抗支糖浆治疗咳嗽变异性哮喘（CVA）的靶点和作用机制。
方法通过中药系统药理学数据库与分析平台（TCMSP）获取抗支糖浆中主要活性成分及其相关靶点，通过在线人类孟德尔遗传数据系统（OMIM）、GeneCards数据库获取CVA相关靶点，采用Venny2.1绘制抗支糖浆靶点与CVA疾病靶点的韦恩图，采用Cytoscape3.6.1软件构建中药〖CD*2〗靶点网络，并应用STRING平台构建蛋白相互作用网络，应用R语言软件对关键靶点进行GO富集分析和KEGG通路富集分析。
结果共获得136个活性成分，160个药物与CVA相关靶点，主要活性成分为豆甾醇、β-谷固醇、槲皮素、儿茶素、木犀草素、山奈酚等。PPI网络中核心基因为蛋白激酶B(AKT1）、白细胞介素（IL）-6、半胱氨酸蛋白酶3（CASP3）、IL-1β、JUN等;GO功能富集后富集数目较多的有受体-配体活性、细胞因子受体结合、细胞因子活性、血红素结合等;KEGG富集通路分析显示抗支糖浆治疗CVA主要通路有脂质与动脉粥样硬化、糖尿病并发症中的AGE-RAGE信号通路以及IL-17信号通路、肿瘤坏死因子信号通路等为主;KEGG通路与基因之间的网络图中关联数量较多的基因主要有IL-1β、NFKBIA、IL-6、CHUK、CXCL8、JUN、CASP3等。
结论初步验证了抗支糖浆的药效基础，主要成分及靶点均显示具有较好的结合性能，可为抗支糖浆治疗CVA提供理论基础。
中文关键词: 网络药理学 抗支糖浆 咳嗽变异性哮喘；活性成分-核心靶点网络
Abstract:ObjectiveToexplorethetherapeutictargetandmechanismofKangzhiSyrupinthetreatmentofcoughvariantasthma（CVA）basedonnetworkpharmacology.
MethodsThemainactiveingredientsandrelatedtargetsinKangzhiSyrupwereobtainedthroughTraditionalChineseMedicineSystemsPharmacology（TCMSP）,andCVArelatedtargetswereobtainedthroughOn-lineMendelianInheritanceinMan(OMIM)andGeneCardsdatabases.Venny2.1wasusedtodrawtheVenndiagramofKangzhiSyruptargetandCVAdiseasetarget,Cytoscape3.6.1softwarewasusedtobuildtheTraditionalChineseMedicine-targetnetwork,STRINGplatformwasusedtoconstructtheproteininteractionnetwork,andRlanguagewasusedtoperformGOenrichmentanalysisandKEGGpathwayenrichmentanalysisonkeytargets.
ResultsAtotalof136activecomponents,and160drug-targetsrelatedtoCVAwereobtained.Themainactivecomponentswerestigmasterol,β-sitosterol,quercetin,（+）-catechin,luteolin,kaempferol,etc.ThecoregenesinPPInetworkincludedAKT1,IL-6,CASP3,IL-1β,JUNetc.AfterGOfunctionenrichment,thereweremorereceptor-ligandactivity,cytokinereceptorbinding,cytokineactivity,hemebindingandsoon.KEGGpathwayenrichmentanalysisshowedthatthemainpathwaysofKangzhiSyrupinthetreatmentofCVAwerelipidandatherosclerosis,AGE-RAGEsignalingpathwayindiabeticcomplications,IL-17signalingpathway,TNFsignalingpathwayandotherpathways.ThegeneswithmoreassociationsinthenetworkmapbetweenKEGGpathwayandgenesincludedmainlyIL-1β,NFKBIA,IL-6,CHUK,CXCL8,JUN,CASP3,etc.
ConclusionThepharmacodynamicbasisofKangzhiSyrupispreliminarilyverified.Themaincomponentsandtargetsshowedgoodbindingproperties,whichcanprovideatheoreticalbasisforthetreatmentofCVAwithKangzhiSyrup.
keywords: Network pharmacology Kangzhi Syrup Cough variant asthma Active component-core target network
文章编号： 中图分类号：R256.12 R562.2+5 文献标志码：A
基金项目:国家自然科学基金（81874485）;黑龙江省自然科学基金（LH2021H088）;黑龙江省中医药科研项目（ZHY2020-149）
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