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Received:November 27, 2021 Published Online:June 20, 2022
Received:November 27, 2021 Published Online:June 20, 2022
中文摘要: 目的 观察骨质疏松性骨折(OPF) 患者血液、骨折断端骨组织中沉默信息调节因子1（Sirt1）基因及蛋白表达变化，探讨Sirt1在OPF发病中的意义。方法 前瞻性选取2018年1月至2021年10月在徐州医科大学附属邳州医院骨科就诊的绝经后女性股骨粗隆间骨折患者50例，其中骨质疏松症患者25例（OPF组），骨量正常患者25例（对照组）。术前采集患者外周血，采用酶联免疫吸附法(ELISA)检测血清中Sirt1浓度。术中于骨折端内用刮匙刮取骨组织约1.0 g，提取骨组织总RNA和总蛋白。采用RT-qPCR方法检测Sirt1的mRNA表达差异；采用Western blot方法检测Sirt1蛋白的表达差异。结果 与对照组相比，OPF组患者血清中Sirt1浓度明显下降，骨组织中Sirt1的RNA及蛋白水平表达均显著降低（P<0.05)。 结论 OPF的发生可能与Sirt1表达降低有关。Sirt1可能是骨质疏松症治疗的一个重要靶标，可通过调控Sirt1的表达干预OPF的发生和治疗。
Abstract:Objective To observe the changes of silent information regulator 1 (SIRT1) gene and protein expression in blood and bone tissue of osteoporotic fracture (OPF) patients, and to explore the significance of SIRT1 in the pathogenesis of OPF. Methods Fifty postmenopausal female patients with femoral intertrochanteric fracture treated in the Department of orthopedics, Pizhou Hospital Affiliated to Xuzhou Medical University from January 2018 to October 2021 were prospectively selected, including 25 patients with osteoporosis (OPF group) and 25 patients with normal bone mass (control group). The peripheral blood was collected before operation, and the concentration of SIRT1 in serum was detected by enzyme-linked immunosorbent assay (ELISA). During the operation, about 1.0 g of bone tissue was scraped with a curette in the fracture end, and the total RNA and total protein of bone tissue were extracted. The mRNA expression of SIRT1 was detected by RT-qPCR, and the expression of SIRT1 protein was detected by Western blot. Results Compared with the control group, the concentration of SIRT1 in serum and the expression of SIRT1 RNA and protein in bone tissue in OPF group decreased significantly (P<0.05). Conclusion The occurrence of OPF may be related to the decrease of SIRT1 expression. SIRT1 may be an important target for the treatment of osteoporosis. The occurrence and treatment of OPF can be intervened by regulating the expression of SIRT1.
文章编号： 中图分类号：R58 R683 文献标志码：A
基金项目:中国国家博士后基金委面上项目(2020M681739)；江苏省卫健委医学科研立项项目(Z2021046)；江苏省重点实验室开放课题(XZSYSKF2020004)；徐州医科大学科技处资助项目(2018KJ23)
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