Abstract:Objective To investigate the difference of five biochemical items in lipemic samples before and after low-temperature high-speed centrifugation, and to evaluate the effect of low-temperature high-speed centrifugation on eliminating the interference of some biochemical indexes. Methods A total of 76 lipemic samples were randomly collected from Nanjing Prevention and Treatment Center for Occupational Diseases from December 2016 to March 2019. Before and after removing lipemia elimination by low-temperature and high-speed centrifugation, the serum samples of lipemia were tested with Hitachi 7600 automatic biochemical analyzer for five biochemical items: total bilirubin (TB), total protein (TP), total cholesterol (TC), triglyceride (TG) and glucose (GLU). The differences and consistency of the test results of these five biochemical items before and after low-temperature high-speed centrifugation were compared. Results There were significant differences in TB, TP, TC and TG before and after lipemia elimination in 76 cases of lipemic samples (P<0.01), but there was no significant difference in GLU (P＞0.05); At the same time, lipid particles had positive deviations on TP, TC and TG, a negative deviation for TB and no obvious deviation for GLU. Conclusion Low-temperature high-speed centrifugation could eliminate the interference of lipid particles on the detection of some biochemical items.