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Received:July 02, 2021 Published Online:February 20, 2022
Received:July 02, 2021 Published Online:February 20, 2022
中文摘要: 目的 研究过敏性紫癜(HSP)患儿外周血单个核细胞(PBMC)中Toll样受体(TLR)2、TLR4的表达及其与滤泡辅助性T细胞(Tfh)免疫应答的相关性。方法 选取2019年5月至10月于徐州医科大学附属医院住院的初发HSP患儿23例作为HSP组,另同时选取于门诊健康体检的儿童15例作为对照组。采用流式细胞术检测两组儿童外周血PBMC中TLR2、TLR4及Tfh细胞的表达,采用ELISA法检测两组儿童血浆中干扰素(IFN)-γ、白细胞介素(IL)-4、IL-6及IL-21水平变化。结果 HSP组患儿外周血中TLR2、TLR4的表达及Tfh细胞比例均明显高于对照组(P<0.01);HSP组患儿血浆中IL-4、IL-6及IL-21水平显著高于对照组,IFN-γ水平和IFN-γ/IL-4比值显著低于对照组,差异有统计学意义(P<0.01)。HSP组急性期患儿外周血中TLR2的表达与Tfh细胞、IL-21水平分别呈显著正相关关系(r=0.538、0.800,P<0.01);TLR4的表达与Tfh细胞、IL-21水平分别呈显著正相关关系(r=0.671、0.660,P<0.01)。结论 TLR2及TLR4活化可能参与了HSP的发生发展,而活化的TLR2及TLR4可能通过上调Tfh细胞免疫应答从而介导HSP的免疫发病机制。
Abstract:Objective To study the levels of toll-like receptor (TLR) 2 and TLR4 in peripheral blood mononuclear cells (PBMC) and their correlation with T follicular helper (Tfh) cell immune response in children with Henoch-Schonlein purpura (HSP). Methods Twenty-three children with primary HSP hospitalized Affiliated Hospital of Xuzhou Medical University from May to October in 2019 were selected as the study subjects (HSP group), and 15 children undergoing physical examination at the same time were served as control group. In two groups, the flow cytometry was used to detect the levels of TLR2, TLR4 and Tfh cells in PBMC, and ELISA was employed to determine the changes in interferon-γ(IFN-γ), interleukin(IL)-4, IL-6 and IL-21 levels in the plasma. Results Compared with those in control group, the expressions of TLR2 and TLR4, the proportion of peripheral Tfh cells and the plasma levels of IL-4, IL-6 and IL-21 in HSP group significantly increased,and IFN-γ level and IFN-γ/IL-4 significantly decreased in HSP group(P<0.01).In the children with acute HSP, the expressions of TLR2(r=0.538,r=0.800) and TLR4 (r=0.671,r=0.660)were respectively positively correlated with Tfh cells and IL-21 levels (P<0.01). Conclusion Activation of TLR2 and TLR4 may be involved in the occurrence and development of HSP and its immune-mediated pathogenesis by up-regulating the immune response of TFH cells.
keywords: Henoch-Schonlein purpura Toll-like receptors T follicular helper cells Interleukin Interferon
文章编号: 中图分类号:R725.9 文献标志码:A
基金项目:
Author Name | Affiliation |
WANG Bin, SHAO Kuan-fu-rong, DONG Chen | Department of Pediatrics, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, China |
Author Name | Affiliation |
WANG Bin, SHAO Kuan-fu-rong, DONG Chen | Department of Pediatrics, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, China |
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