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Received:June 11, 2021 Published Online:October 20, 2021
Received:June 11, 2021 Published Online:October 20, 2021
中文摘要: 目的 探讨不稳定型心绞痛患者外周血单个核细胞（PBMC）中miR-132和单核细胞趋化蛋白-1（MCP-1）的表达及其与冠状动脉（冠脉）病变严重程度的相关性。
方法 收集2018年10月至2019年2月于西安交通大学第一附属医院住院患者共216例，其中不稳定型心绞痛（UAP）126例，非冠心病（NCHD）90例。用密度梯度离心的方法分离PBMC，RT-qPCR检测miR-132和MCP-1 mRNA相对表达量。比较miR-132和MCP-1 mRNA的表达分别与冠脉病变血管支数和Gensini评分的相关性。
结果 UAP组miR-132及MCP-1 mRNA表达水平均显著高于NCHD组（ P <0.01）。所有患者PBMC中MCP-1 mRNA与miR-132表达水平呈正相关（ r =0.706， P <0.01）。根据冠脉病变血管支数和Gensini评分，将UAP患者分为单支、双支及三支病变亚组（ n =42、47、37）和低分、中分及高分亚组（ n =45、41、40）。与单支亚组相比，双支及多支亚组miR-132及MCP-1表达水平均增高（ P <0.05）。与低分亚组相比，中分和高分亚组miR-132及MCP-1 mRNA表达水平均增高（ P <0.01）。Gensini评分分组与miR-132（ r =0.619， P <0.01）和MCP-1 mRNA表达水平（ r =0.623， P <0.01）均呈正相关。ROC曲线分析示，对冠脉严重病变的诊断，miR-132的AUC为0.720（95% CI ：0.624～0.815， P <0.01），MCP-1的AUC为0.742（95% CI ：0.647～0.837， P <0.01），均有较高诊断效能。
结论 UAP患者PBMC中miR-132表达水平升高，并且与炎症因子MCP-1水平呈正相关。UAP患者PBMC中的miR-132和MCP-1水平有望作为冠脉病变严重程度的预测指标。
中文关键词: 不稳定型心绞痛 外周血单个核细胞 微小核糖核酸-132 单核细胞趋化蛋白-1 冠状动脉病变程度
Abstract:Objective To investigate the expression of miR-132 and monocyte chemoattractant protein-1 (MCP-1) in peripheral blood mononuclear cells (PBMC) and their correlation with the severity of coronary artery disease in patients with unstable angina pectoris （UAP）.
Methods A total of 216 patients admitted to the First Affiliated Hospital of Xi'an Jiaotong University from October 2018 to February 2019 were collected, among them 126 were UAP (UAP group) and 90 were non-coronary heart disease(NCHD group). PBMC were isolated by density gradient centrifugation, and the relative expression level of miR-132 and MCP-1 mRNA were detected by RT-qPCR. The correlations of the expression of miR-132 and MCP-1 mRNA with the number of diseased coronary arteries or Gensini score were compared.
Results The expressions levels of miR-132 and MCP-1 mRNA in UAP group were significantly higher than those in NCHD group ( P <0.01). There was a positive correlation between the expression levels of MCP-1 mRNA and miR-132 in PBMC of all patients ( r =0.706, P <0.01). According to the number of diseased coronary arteries and Gensini score, patients with UAP were divided into single-vessel, two-vessel and three-vessel lesion subgroup ( n =42,47,37) and low-, middle- and high-score subgroup ( n =45,41,40). Compared with single-vessel subgroup, the expression levels of miR-132 and MCP-1 in the two-vessel and three-vessel lesion subgroup were significantly higher( P <0.05). Compared with the low-score subgroup, the expression levels of miR-132 and MCP-1 mRNA in middle- and high -score subgroup were significantly higher ( P <0.01). Gensini score grouping was positively correlated with the expression levels of miR-132 ( r =0.619， P <0.01) and MCP-1 ( r =0.623, P <0.01). ROC curve analysis showed that for the diagnosis of severe coronary lesions, the AUC of miR-132 was 0.720 (95% CI : 0.624-0.815, P <0.01), and the AUC of MCP-1 was 0.742 (95% CI : 0.647-0.837, P <0.01), all of which had high diagnostic efficiency.
Conclusions The expression level of miR-132 in PBMC of UAP patients is increased and positively correlated with the level of inflammatory cytokine MCP-1. The levels of miR-132 and MCP-1 in PBMC of UAP patients are expected to be used as predictors of coronary lesion severity.
keywords: Unstable angina pectoris Peripheral blood mononuclear cell Micro ribonucleic acid-132 Monocyte chemoattractant protein-1 Severity of coronary artery disease
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基金项目:陕西省2019年重点研发计划（2019SF-164）
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