Abstract:Objective To analyze the antibiotic resistance of food-borne pathogens in Nanjing and provide the basis for the prevention of food-borne diseases and the guidance of clinical rational drug use.Methods The broth microdilution method was used to identify the drug sensitivity of 85 strains of Salmonella,Vibrio parahaemolyticus and diarrheogenic Escherichia coli isolated from diarrhea samples of food-borne diseases patients treated in Nanjing from January 2019 to December 2019.Results Before drug sensitivity test,85 strains were confirmed to be positive by biochemical identification,and the multiple drug resistance rates of Salmonella (35 strains),Vibrio parahaemolyticus (36 strains) and diarrheogenic Escherichia coli (14 strains) were 65.7%,0% and 78.6% respectively.The main antibiotics in drug-resistant strain of salmonella were erythromycin (100.0%),ampicillin/sulbactam (65.7%),cefazolin (65.7%) and nalidixic acid (65.7%),ampicillin (62.9%).Cefazolin (100.0%),ampicillin (11.1%) and gentamicin (2.8%) were the main antibiotics in drug-resistant strain of Vibrio parahaemolyticus.The main antibiotics in drug-resistant strain of diarrheogenic Escherichia coli were erythromycin (100.0%),nalidixic acid (71.4%),ampicillin (64.3%),ampicillin/sulbactam(57.1%),azithromycin(57.1%).conclusions Multiple antibiotic resistance in diarrheogenic Escherichia coli and Salmonella isolated from fecal samples of food-borne diseases patients should be continuously monitored to understand the isolation and drug resistance of food-borne pathogens in Nanjing and to guide the rational use of antibiotics in this area.