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Received:January 13, 2021 Published Online:July 20, 2021
Received:January 13, 2021 Published Online:July 20, 2021
中文摘要: 目的讨论亚甲基四氢叶酸还原酶(MTHFR)基因位点A1298C多态性分布及同型半胱氨酸(Hcy)水平与青海藏族原发性高血压(EH)的关联性。方法整群抽样收集2015至2016年青海省人民医院心血管内科住院的藏族EH患者60例为试验组(EH组),同一时期门诊体检的无EH青海藏族人群75例为对照组(非EH组)。收集一般资料,测定Hcy、SBP、DBP、TC、TG、LDL-C、BMI水平;聚合酶链反应-限制性片段长度多态性技术（PCR-RFLP）测定 MTHFR A1298C的基因分布,观察其在青海藏族的分布特征,及Hcy水平在各基因型间的差异,分析MTHFR A1298C分布及Hcy与EH有无相关性。结果青海藏族EH组Hcy水平高于非EH组［(19.02±8.31) μmol/L vs (15.65±5.76) μmol/L,t=2.712,P=0.008］。EH组和非EH组MTHFR A1298C三种基因型AA、AC、CC频率分布(P=0.563),等位基因A、C频率分布(P=0.873),以及AA、AC＋CC频率分布(P=0.583)均无统计学差异。EH组和非EH组MTHFR A1298C三种基因型AA、AC、CC的Hcy水平比较,差异无统计学意义(P=0.233)。Logistic回归分析结果示,高BMI(OR=7.160,95%CI:2.925～17.527,P<0.01)为EH的独立危险因素;高Hcy血症不是EH的独立危险因素(OR=1.567,95%CI:0.513～4.788,P>0.05)。结论虽然青海藏族EH患者Hcy水平较非EH人群高,但是,高Hcy血症并非青海藏族人群EH的独立危险因素。青海藏族人群中MTHFR A1298C基因突变与Hcy水平和高血压的发生均无明显相关性,A1298C突变可能不是EH的危险因素。
中文关键词: 原发性高血压 藏族 亚甲基四氢叶酸还原酶基因 多态性 同型半胱氨酸
Abstract:Objective To investigate the associations of distribution of methylenetetrahydrofolate reductase (MTHFR) gene A1298C polymorphisms and homocysteine (Hcy) level with essential hypertension (EH) in Qinghai Tibetans.
Methods The cluster sampling method was used to enroll 60 Tibetan EH patients hospitalized in Cardiovascular Department of Qinghai Provincial People′s Hospital from 2015 to 2016 as case group(EH group),and in the same period,75 Tibetan people without EH were selected as control group(non-EH group).The levels of Hcy,systolic blood pressure(SBP),diasolic blood pressure (DBP),serum triglyceride (TG),total cholesterol (TC),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C) and body mass index (BMI) were measured.MTHFR A1298C gene polymorphisms were detected by polymerase chain enzyme restriction fragment length polymorphism (PCR-RFLP) method to observe its distribution characteristics in Qinghai Tibetans and the differences of Hcy levels among different genotypes.The associations of distribution of MTHFR A1298C and Hcy level with EH were analyzed.
Results The Hcy level in EH group was higher than that in non-EH group ［(19.02±8.31) μmol/L vs (15.65±5.76) μmol/L,t=2.712,P=0.008］.There was no significant difference in genotypes frequency of AA,AC,CC of MTHFR A1298C (P=0.563),A and C allele frequency for MTHFR A1298C(P=0.873)and frequency distribution of AA,AC plus CC genotype (P=0.583) between EH group and non-EH group.There was no significant difference in Hcy levels for AA,AC and CC genotypes of MTHFR A1298C between two groups (P=0.233).Logistic regression analysis showed that high BMI (OR=7.160,95%CI:2.925-17.527,P＜0.01)was an independent risk factor for EH,but hyperhomocysteinemia (HHcy) was not an independent risk factor for EH (OR=1.567,95%CI:0.513-4.788,P＞0.05).
Conclusions Although Hcy level of Tibetan EH patients is higher than that in normal Tibetan people,HHcy is not an independent risk factor for EH in Qinghai Tibetan people.A1298C mutation of MTHFR gene and Hcy level are not correlated to EH.A1298C mutation may be not the risk facter of EH.
keywords: Essential hypertension Tibetan Methylenetetrahydrofolate reductase gene Polymorphism Homocysteine
文章编号： 中图分类号：R544.1 文献标志码：A
基金项目:青海省科技创新能力促进计划项目“西部之光”(青科发计财［2014］141)
| Author Name | Affiliation |
| CHEN Dan-dan*,CHANG Rong | *Electrocardiographic Room,Luoyang Central Hospital Affiliated to Zhengzhou Uninersity,Luoyang,Henan 471000,China |
| 常荣2 | 2.青海省人民医院心血管内科,青海 西宁810000 |
| Author Name | Affiliation |
| CHEN Dan-dan*,CHANG Rong | *Electrocardiographic Room,Luoyang Central Hospital Affiliated to Zhengzhou Uninersity,Luoyang,Henan 471000,China |
| 常荣2 | 2.青海省人民医院心血管内科,青海 西宁810000 |
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