Abstract:Objective To study the levels and their correlations of apocrine secretion odor-binding protein (ASOB),apolipoprotein D (ApoD) and angiotensin II receptor 2 (AngⅡ2R) in axillary apocrine sweat gland. Methods A total of 92 patients (40 males and 52 females) with axillary osmidrosis(AO) receiving surgical treatment from January 2018 to January 2020 were selected as AO group,in which osmidrosis was mild in 31,moderate in 43 and severe in 18 patients.During the same period,50 female patients undergoing breast augmentation through the axillary approach were served as control group.The mRNA and protein expressions of ASOB,ApoD and AngⅡ2R were respectively detected by fluorescent quantitative PCR and immunohistochemistry(IHC).Pearson linear correlation was used to analyze the correlations among them. Results IHC results showed that ApoD and AngⅡ2R protein expressions were positive with brownish yellow or brown in the cytoplasm of apocrine sweat gland cells of AO patients,and ASOB protein expression was positive with brownish yellow.Compared with control group,the mRNA expression levels of ASOB,ApoD and AngⅡ2R significantly increased in AO group (all P<0.01).Compared with the patients with AO at rest period,the mRNA expression levels of ASOB,ApoD,and AngⅡ2R increased statistically in the patients with AO at active period (all P<0.01).The mRNA expression levels of ASOB,ApoD,and AngⅡ2R significantly increased with the increase of degree of axillary osmidrosis (all P<0.01).Correlation analysis showed that there were positive correlations between ASOB and ApoD (r=0.341,P=0.001),between ApoD and AngⅡ2R (r=0.525,P=0.001) and between ASOB and AngⅡ2R (r=0.474,P=0.001). Conclusions ASOB,ApoD and AngⅡ2R are highly expressed in the apocrine sweat glands of AO and are associated with different secretion periods and degrees.The positive correlations among ASOB,ApoD,and AngⅡ2R (by pairwise comparison) suggest that they play an important participation in the apocrine sweat glands of AO.