Abstract:Objective To analyze the incompatibility cases in cross matching blood test to provide evidence for precise blood transfusion.Methods From January 2014 to June 2019 in Nanjing Jiangbei People′s Hospital,97 samples of cross-matching incompatibility were tested for blood transfusion compatibility,irregular antibody screening and antibody specificity identification by method of purifying brine,microcolumn gel test and anti-human globulin test.Results In 97 samples of crossmatch incompatibility,73 cases (75.26%) were mismatched between the main sides and 24 cases (24.74%) between the second sides;87 cases (89.69%) were caused by recipient factors,and 10 cases (10.31%) were from donor factors.In 87 incompatible recipients,Rh blood group system accounted for 47.13%,warm autoantibodies accounted for 26.44%,cold autoantibodies accounted for 10.34%,other blood group system accounted for 12.64% and ABO blood group subtype was 3.45%.Conclusions Irregular antibodies and warm autoantibodies produced by Rh blood group system are the main causes of mismatching.In the presence of alloantibodies,the corresponding red blood cells with antigen negative should be selected for homotypic transfusion.