Abstract:ObjecitveTo investigate the effect of immune conditioning on intestinal barrier after diffuse brain injury(FBI), and its protective effect on intestinal barrier. Methods The FBI model of adult male Wistar rats was reproduced by marmarmarou method.The injured and resuscitated rats were divided into model group, ulinastatin (UTI)group in addition, the rats with only the scalp incision without injury were used as the control group.The levels of diamine oxidase (DAO), tumor necrosis factor (TNF)-α and interleukin (IL)-10 were observed at 3, 6, 12, 24 and 48 hours after injury, and the changes of intestinal villi height were observed under light microscope. Results Compared with the control group, the plasma DAO content in the model group and UTI group was significantly higher, while the height of intestinal villi was significantly lower.The difference was statistically significant at 6 hours and 3 hours after injury, respectively (P<0.05).Compared with the model group, the DAO content in UTI group decreased significantly with time coursing, while the villus height increased significantly.The difference between the two groups began to appear after 12 hours in DAO and 24 hours in villus height (P<0.05).The level of TNF-α and IL-10 in UTI group and model group were significantly higher than those in the control group, but the UTI group decreased more significantly than the model group, while the IL-10 level of UTI group was higher than that of model group (all P<0.05). Conclusion Ulinastatin can reduce the level of TNF-α in blood, increase the level of IL-10, reduce the inflammatory response, promote the repair of intestinal mucosa, and show the immunomodulatory effect in the early stage of trauma.