Abstract:Objective To explore the significance of irregular antibody detection before blood transfusion for safe use of blood in clinic. Methods A total of 1 600 patients needing blood transfusion in the Affiliated Hospital of Yan′an University from January 2018 to January 2019 were selected as the subjects.Before blood transfusion,the irregular antibody was identified using Coombs card,and then cross matching experiment was carried out to observe the detection results of patients. Results Of the 1 600 patients,there were 950 male and 650 female,and 15 were positive for irregular antibody(0.9%),including 3 males and 12 females. The positive rate of irregular antibodies in males was significantly lower than that in females（0.3% vs 1.8%，χ²=9.733,P<0.01).In 15 patients with irregular antibody positive,the test results showed that 7 patients were Rh blood group system,followed by 3 patients with MNS blood group system and 1 patients with Kidd,Duffy,Lewis,Diego,Do,respectively.Blood cross matching test in 15 patients showed no hemolytic transfusion reaction.Specificity test determined specific antibodies in 10 cases(anti-D 1,anti-E 3,and negative anti-Ce 1,anti-Ee 1,anti-JKh 1,anti-Fya 1,anti-Lea 1 and anti-Mur 1),and 5 cases had no specific antibodies. ConclusionThe detection of irregular antibody before blood transfusion is helpful to clinical safe blood use,effectively avoid hemolytic transfusion reaction caused by antibody.