Abstract:Objective To detect and analyze the distribution of bacteria in urine culture from 2015 to 2017 and the drug sensitivity test in vitro so as to provide the reference for clinical rational use of drugs.? Methods The bacterial distribution cultured in urine and the monitoring of drug resistance from January 1, 2015 to January 1, 2017 were analyzed retrospectively. The identification of pathogenic bacteria was madeby API identification system from French bioMerieux company. The drug susceptibility test was performed by disk diffusion(k-b) method.? Results Out of 627 urine culture specimens, pathogenic bacteria were isolated from 558 cases (34.3%) in which there were Escherichia coli pathogen (256 strains, 45.9%), being the most common, Enterococcus faecium(67 strains, 12%), Klebsiella pneumoniae (45 strains, 7.7%), copper Pseudomonas aeruginosa(37 strains, 6.6%), Enterococcus faecalis (32 strains, 5.7%). Escherichia coli produced extended spectrum beta lactamase (ESBL) was 63.3%, and ESBL produced by Klebsiella pneumoniae was 31.5%. Vancomycin-and linezolid-resistant strains were not found among staphylococcus.? Conclusion The main pathogens of urinary tract infection are Escherichia coli and Enterococcus, and antibiotics should be selected based on the results of antibiotic susceptibility, so as to reduce the emergence of drug-resistant strains.