Abstract:Objective To investigate the changes of four and a half LIM domains protein 2 (FHL2) level and the changes of related molecular biologic characteristics in epithelial-mesenchymal transition(EMT) by establishing EMT model of SGC-823 gastric carcinoma cells.? Methods The human SGC-823 gastric carcinoma cells were cultured in vitro (control group) and were induced into the cells of resistance to oxaliplatin (L-OHP) by L-OHP constant stimulation method to establish EMT model (experiment group). The inverted phase contrast microscope was used to observe the cellular morphology. Transwell invasion experiment was used to detect cell invasion ability. Western blot experiment was used to detect the expressions of E-cadherin, vimentin and FHL2.? Results Compared with control group, the cell phenotype in experimental group was converted to phenotype of interstitial like cells; the number of cells passing through the membrane was higher(65.7±11.65 vs 123.4±28.13, t=12. 434, P<0.01); E-cadherin expression level was down- regulated (1.47±0.13 vs 0. 76±0.1) ; vimentin (0. 65±0. 21 vs 1. 01±0. 25)and FHL2 (0. 34±0.09 vs 0. 71±0.11) expression levels were up-regulated in experiment group(all P<0.05).? Conclusion After stimulation of L-OHP, EMT occurres in SGC-823 gastric carcinoma cells, namely, the phenotype of SGC-823 cells is changed from epithelial like cells to interstitial cells which will has stronger invasion ability, at the same time, down-regulation of E-cadherin expression and up-regulation of vimentin and FHL2 expressions are accompanied.