Abstract:Objective To observe the effect of statins of different doses on the level and function of circulating endothelial progenitor cells (EPCs) in patients with unstable angina pectoris and explore the mechanism of getting benefit from statins intensive therapy in preoperative period of percutaneous coronary intervention (PCI). Methods A total of 128 patients with unstable angina pectoris admitted in Cardiology Department of Affiliated Hospital of Qingdao University were selected. The patients were randomly divided into experiment group(n=64) and control group(n=64). Different doses of rosuvastatin were respectively given 1 day before PCI in experiment group (20 mg) and control group (10 mg). According to the results of coronary angiography after PCI, 60 patients met inclusion and exclusion criteria were screened out (n=30, each group). Collecting venous blood at 1-day before operation, 24-hour and 7-day after operation, isolation, culture and identification of EPCs in blood samples were performed. Fluorescence staining was used to count EPCs. methyl thiazolyl tetrazolim (MTT)colorimetry and modified Boyden chamber assay were used to respectively detect the abilities of EPCs proliferation and migration. Results The number of EPCs 24 h after operation in experiment group was significantly more than that in control group (88.90±7.230 vs 85.10±6.567, P<0.05).There were no significant differences in number of EPCs migration (50.33±5.659 vs 47.67±6.397, P>0.05) and OD value (0.406±0.059 vs 0.380±0.074, P>0.05) 24 h after operation between two groups. The number of EPCs 7 days after operation in experiment group was significantly more than that in control group (79.07±8.542 vs 70.03±9.514, P<0.01). The number of EPCs migration (46.67±5.990 vs 42.53±6.653, P<0.05) and OD value (0.789±0.072 vs 0.719±0.091, P<0.01) 7 days after operation in experiment group were significantly more than those in control group. Conclusion Compared with conventional dose, enhanced treatment of 20 mg rosuvastatin in PCI perioperative period can significantly increase the number of postoperative EPCs and proliferation and migration functioins of EPCs which may be one of the mechanisms getting benefit for enhancing statins therapy in the perioperative period.