Abstract:Objective To investigate the clinical curative effect of Wenyang Jianpi decoction on spleen-kidney yang deficiency type laxative constipation and its effect of stem cell factor(SCF)/c-kit signaling pathway. Methods One hundred and sixty spleen-kidney yang deficiency type laxative constipation patients who were hospitalized from January 2015 to December 2016 were selected, and all the patients were divided into control group and study group randomly (n=80). Patients in control group were given mosapride oral, each time 5 mg and 3 times a day. The study group was given Wenyang Jianpi decoction. Both groups were treated for 4 weeks. After the course of treatment, apart and total tradition Chinese medical (TCM) symptom score and clinical curative of each group were compared. At the same time, western bolt was used to detect the expression level of SCF and c-kit protein in the colon tissues which were taken by colonoscopy. Results After the treatment, the defecation difficulty, stool property, defecation time, defecation interval and total TCM symptom score in both groups were all significantly lower than those before the treatment, and all these indexes in the study group was lower than the control group (P＜0.05, P＜0.01). The clinical curative effect in the study group was significant higher than that in control group (Z=5.56, P＜0.05). Expression level of SCF and c-kit protein in the study group was higher than the control group after the treatment (P＜0.05). In the study group, expression level of SCF and c-kit protein were higher after the treatment (P＜0.05), while there was no statistically difference before and after the treatment in the control group (P＞0.05). Conclusion Using Wenyang Jianpi decoction in the treatment of spleen-kidney yang deficiency type laxative constipation patients could improve patient′s clinical symptoms and clinical curative effect. It may be related to the high expression level of SCF and c-kit protein and the improvement of SCF/c-kit signaling pathway by Wenyang Jianpi decoction.