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投稿时间：2024-03-12 网络发布日期：2025-05-20
投稿时间：2024-03-12 网络发布日期：2025-05-20
中文摘要: 目的 探讨营养不良儿童肠道菌群的状况及其与血清中炎症细胞因子、 25羟维生素 D［25（OH）D］水平之间的关系。方法 选取 2022年 1月至 2023年 8月在宁德市医院就诊的 126例营养不良儿童作为研究组，另选取同期在同院行健康体检的儿童 133例作为对照组。收集所有研究对象的临床资料；采用菌群形成单位（CFU）计数法统计两组对象粪便中肠球菌、大肠杆菌、乳酸菌和双歧杆菌菌落总数的对数值；酶联免疫吸附测定（ELISA）法检测血清肿瘤坏死因子-α（TNF-α）、 C反应蛋白（CRP）、白细胞介素 -6（IL-6）及 25（OH）D水平；以 Pearson相关分析法分析 CRP、IL-6、TNF-α、25（OH）D与肠道菌群的相关性。结果 研究组身体质量指数低于对照组，有消化道病史比例高于对照组（P<0.05）。与对照组相比，研究组肠球菌、大肠杆菌菌落数较高，双歧杆菌、乳酸杆菌菌落数较低， TNF-α、CRP、IL-6水平较高，血清 25（OH）D水平较低（P<0.05）。 Pearson分析结果显示营养不良儿童的大肠杆菌、肠球菌分别与 TNF-α、CRP、IL-6水平呈正相关，与 25（OH）D水平呈负相关（P<0.05）。双歧杆菌、乳酸杆菌分别与 TNF-α、CRP、IL-6呈负相关，与 25（OH）D呈正相关（P<0.05）。结论 血清 CRP、IL-6、TNF-α、25（OH）D水平与营养不良儿童的肠球菌、大肠杆菌、乳酸菌和双歧杆菌菌群失衡密切相关。
Abstract:Objective To investigate the status of intestinal flora in malnourished children and its relationship with se.rum inflammatory cytokines and 25-hydroxyvitamin D［25 (OH) D］levels. Methods A total of 126 malnourished children admitted to Ningde Affiliated Hospital from January 2022 to August 2023 were selected as the study group. Additionally, 133 healthy children undergoing check.ups at the same hospital during the same period were selected as the control group. The clinical data of all study subjects were collected. Colony forming unit (CFU) counting method was used to count the total number of Enterococcus, Escherichia coli, Lactobacillus , and Bifidobacterium in the feces of the research subjects, and their logarithmic value were recorded. Enzyme-linked immunosorbent assay (ELISA) was used to measure serum levels of tumor necrosis factor-α (TNF-α) , C.reactive protein (CRP) , interleukin-6 (IL-6) , and 25 (OH) D. Pearson correlation analysis was employed to analyze the correlation between CRP, IL-6, TNF-α, 25 (OH) D, and gut microbiota. Results Children in the study group had lower body mass index (BMI) and a higher incidence of gastrointestinal diseases compared to the control group (P＜0.05) . Compared to the control group, the study group showed higher counts of Clostridium and Escherichia coli, lower counts of Bifidobacterium and Lactobacillus, higher levels of TNF-α, CRP, IL-6, and significantly lower serum 25 (OH) D levels (P＜0.05) . Pearson correlation analysis indicated that Escherichia coli and Clostridium in malnourished children were positively correlated with TNF-α, CRP, IL-6 levels, and negatively correlated with 25 (OH) D levels, respectively (P＜0.05) . Bifidobacterium and Lactobacillus were negatively correlated with TNF-α, CRP, IL-6 levels, and positively correlated with 25 (OH) D levels, respectively (P＜ 0.05). Conclusion CRP, IL-6, TNF-α, and serum 25 (OH) D levels are closely associated with Clostridium, Escherichia coli, Bifidobacterium and Lactobacillus in malnourished children.
keywords: Malnourished children Gut microbiota Serum 25-hydroxy vitamin D Clostridium Escherichia coli Bifidobacterium Lactobacillus
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| Author Name | Affiliation |
| ZHENG Jinlu, WANG Jingjing, LI Haiqing, CHEN Xiangjian | Department of Pediatrics, Ningde Hospital Affiliated to Ningde Normal University, Ningde, Fujian 352100, China |
引用文本：
郑金炉，王晶晶，李海青，陈向坚.血清炎症指标及25羟维生素D水平与营养不良儿童肠道菌群的相关性[J].中国临床研究,2025,38(5):763-766.
郑金炉，王晶晶，李海青，陈向坚.血清炎症指标及25羟维生素D水平与营养不良儿童肠道菌群的相关性[J].中国临床研究,2025,38(5):763-766.