Abstract:Objective To investigate the potential mechanism of action of Buzhong Yiqi Decoction in the treatment of cervical human papillomavirus (HPV) infection based on network pharmacology and molecular docking technology. Methods The main chemical components and action targets of Buzhong Yiqi Decoction were searched and screened from TCMSP databases, and the gene names were unified by Uniprot database. Target genes related to cervical HPV infection diseases were collected from OMIM, GeneCards, DisGeNET and other databases. Wayne analysis was carried out to get the potential target of Buzhong Yiqi Decoction in treating cervical HPV infection, and the protein-protein interaction (PPI) network of Buzhong Yiqi Decoction in treating cervical HPV infection was constructed by using STRING database, and the topological analysis was carried out. GO and KEGG analysis were performed on the cross genes of drugs and diseases. The key active components and main target genes of the drug obtained before were imported into Autodockvina software for molecular docking test. Results Finally, 256 active components of Buzhong Yiqi Decoction were screened out, including 155 genes related to cervical HPV infection. PPI analysis suggested that the core target genes of Buzhong Yiqi Decoction in treating cervical HPV infection diseases were AKT1, TP53, IL6, VEGFA, JUN, etc. GO and KEGG analysis showed that key targets involved a variety of molecular function affecting protein binding, identical protein binding, enzyme binding etc, acting on a variety of signaling pathways such as TNF, IL-17 and AGE-RAGE signaling pathway, etc. The molecular docking results showed good docking activity between the ligand and the receptor. Conclusion Buzhong Yiqi Decoction intervenes in multiple pathways by affecting multiple targets to achieve therapeutic effects on cervical HPV.