Abstract:ObjectiveTo investigate the clinical diagnostic efficacy of color Doppler ultrasound (CDU) combined with serum carbohydrate antigen (CA) 125, cysteine-rich protein 61 (CYR61) and insulin-like growth factor-1 (IGF-1) for breast cancer. Methods Eighty six female breast cancer patients treated in Hanyang Hospital Affiliated to Wuhan University of Science and Technology from January 2021 to December 2023 were included in the breast cancer group, and 86 female benign breast disease patients treated in the same period were included in the benign group for retrospective analysis. CDU and serum CA125, CYR61, IGF-1 were detected in both groups. The CDU characteristics and serum CA125, CYR61 and IGF-1 levels of patients in the two groups, and those of breast cancer patients with different TNM grades were analyzed. ROC curve was used to analyze the diagnostic efficacy of CDU, serum CA125, CYR61 and IGF-1 for breast cancer alone and in combination. Results Compared with benign group, the proportions of unclear boundaries, irregular morphology, microcalcification, uneven internal echo, and high-grade blood flow signals, as well as resistance index (RI), pulsatility index (PI), 〖JP2〗and peak systolic velocity (PSV) values in breast cancer group were higher (P＜0.05）.The levels of serum CA125, 〖JP+1〗CYR61, and IGF-1 all increased in breast cancer group〖JP〗 compared〖LM〗 to benign group (P＜0.01). ROC curve analysis showed that the area under the curve (AUC) of CDU, serum CA125, CYR61, IGF-1 and their combined diagnosis of breast cancer were 0.874, 0.832, 0.849, 0.806 and 0.962, respectively，with the highest AUC, specificity (95.29%) and sensitivity (93.86%) in combined diagnosis of four indexes. Conclusion CDU combined with serum CA125, CYR61 and IGF-1 has a high clinical value in the diagnosis of breast cancer.