本文已被:浏览 1482次 下载 377次
投稿时间:2022-03-30 网络发布日期:2023-01-20
投稿时间:2022-03-30 网络发布日期:2023-01-20
中文摘要: 目的 通过动物实验观察褪黑素(MT)对缺血再灌注后大鼠肾脏线粒体氧化应激及炎症状态的影响。
方法 60只SD大鼠随机分为假手术组(Sham组, n =10)、缺血再灌注组(IR组, n =25)及缺血再灌注+MT 3.0 mg/kg组(MT 3.0 mg 组, n =25)。建立再灌注模型,于缺血再灌注6、24 h后留取静脉血检测血清尿素氮(BUN)、血清肌酐(Scr)水平;取一侧肾脏上极制作HE染色标本用于观察肾脏组织结构改变(并作Paller法肾小管损伤程度评分)和免疫组化法检测肿瘤坏死因子-α(TNF-α)及白介素6(IL-6)蛋白表达;取另一侧肾皮质制备匀浆、提取肾组织线粒体,用酶联免疫吸附法检测线粒体中TNF-α、高敏C反应蛋白(hs-CRP)、IL-6、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)水平。
结果 缺血再灌注6 h及24 h后,与Sham组各时点比较,IR组BUN、Scr水平,Paller评分,肾脏组织IL-6、TNF-α表达及肾脏线粒体中hs-CRP、TNF-α、IL-6、MDA水平均升高( P <0.01),GSH-Px、SOD水平均降低( P <0.01);与IR组各时点比较,加MT后的MT 3.0 mg组上述各指标均呈现反向变化,差异均有统计学意义( P <0.01)。
结论 MT能改善缺血再灌注时肾脏线粒体氧化应激及炎症状态,有效缓解缺血再灌注所致的大鼠肾脏损伤并改善肾功能。
Abstract:Objective To observe the effects of melatonin(MT) on oxidative stress and inflammation state of renal mitochondria after renal ischemia reperfusion injury(IRI) in rats.
Methods Sixty SD rats were randomly divided into sham-operation group(Sham group, n =10), ischemia reperfusion group(IR group, n =25) and ischemia reperfusion plus MT(3.0 mg/kg) group(MT 3.0 mg group, n =25). At 6- and 24-hour after successful establishment of ischemia-reperfusion model, venous blood was taken to detect the levels of serum urea nitrogen(BUN) and serum creatinine(Scr). The upper pole of one kidney was taken for HE staining specimen to observe the renal histomorphological changes(the score of renal tubule injury degree evaluated by Paller method) and the protein expressions of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) by immunohistochemistry. The renal cortex homogenate from the other unilateral kidney was prepared for extracting mitochondria, which was used for the detection of the levels of TNF-α, high-sensitive C-reactive protein(hs-CRP), IL-6, malondialdehyde(MDA),glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) by Enzyme linked immunosorbent assay.
Results After 6- and 24-hour of ischemia-reperfusion, compared with those in Sham group at each time point, the levels of BUN and Scr, Paller score, the expressions of IL-6 and TNF-α in renal tissue and the levels of hs-CRP,TNF-α, IL-6 and MDA in renal mitochondria significantly increased( P <0.01), and the levels of GSH-Px and SOD decreased in IR group( P <0.01); compared with those in IR group at each time point, the above indicators showed reverse changes in MT 3.0 mg group( P <0.01).
Conclusion MT can attenuate the oxidative stress and inflammatory of renal mitochondria during ischemia-reperfusion, effectively alleviate renal IRI and improve renal function.
文章编号: 中图分类号:R692 文献标志码:A
基金项目:武汉市卫生健康委医学科研面上项目(WX19C37)
Author Name | Affiliation |
YI Ye, HU Ting-ting, WANG Ying, HUANG Wei, YU Ya, CHEN Jian-wu | Department of Nephrology, The Third Peoples Hospital of Hubei Province, Wuhan, Hubei 430033, China |
引用文本:
易晔,胡婷婷,王莹,黄薇,余雅,陈建武.褪黑素对大鼠缺血再灌注肾脏线粒体氧化应激及炎症状态的影响[J].中国临床研究,2023,36(1):18-22.
易晔,胡婷婷,王莹,黄薇,余雅,陈建武.褪黑素对大鼠缺血再灌注肾脏线粒体氧化应激及炎症状态的影响[J].中国临床研究,2023,36(1):18-22.