Abstract:The emergence of multi-drug resistance makes bacterial infection a major threat to public health and economy. The formation of bacterial biofilm is one of the main reasons for bacterial resistance. The complexity of chemical composition and physical structure of biofilm makes the removal of mature biofilm a difficult problem. Due to the high antibiotic resistance of biofilm, effective antibacterial agents and new anti-biofilm strategies are urgently needed. Researchers have done a lot of research work in this field. This paper reviews the current strategies in eliminating mature biofilm and inhibiting biofilm formation in order to provide appropriate anti-biofilm strategies for clinic.