Abstract:Objective To study the relationship between KIAA1324 gene and proliferation, migration and invasion in human pancreatic cancer cell, and to explore the possible mechanism of invasion and metastasis in pancreatic cancer. Methods KIAA1324 gene was over-expressed by culturing human pancreatic cancer cells called line PANC-1 cells, building KIAA1324 over-expressive plasmids and transfecting PANC-1 cells, and then target gene over-expressed was verified by qPCR experiments. The experiment was divided into 3 groups, blank control group (CON group), negative control group (NC-OE group) and gene over-expression group (KIAA1324-OE group). Cell proliferation level was detected by Celigo and CCK-8 method, cell migration ability was detected by scratch experiment, cell invasion ability was detected by Transwell experiment, and then the differences in cell proliferation, migration and invasiveness among three groups were compared by the above methods. Results The cell proliferation ability, migration ability and invasion ability in KIAA1324-OE group were higher than those in CON group and NC-OE group, the difference was statistically significant (P<0.05).Conclusions Over-expression of KIAA1324 gene can increase the proliferation, migration and invasion ability of PANC-1 cells in pancreatic cancer.