Abstract: Objective To explore the implementation effect of list management system on infection prevention and control in novel coronavirus pneumonia (COVID-19) hospital. Methods Seventy-five professional nursing staffs in four isolation wards (D1, D2, D4, D5) of Nanjing Public Health Center in August 2021 were selected as the research objects for the implementation of list management. Routine management was used in control group, and list management was conducted in experimental group. The implementation effect of list management was evaluated based on the supervision and hospital management, qualified rate of wearing and taking off protective clothing, hand hygiene compliance and accuracy analysis, environmental monitoring and analysis, on-site disposal after occupational exposure and efficiency and satisfaction of infection control. Results The scales for supervision and management increased significantly in experimental group compared with those in control group (P<0.05). The qualified rates of wearing and taking off protective clothing reached 97.31% (1 121/1 152) and 96.09% (1 107/1 152) respectively, and there was no nosocomial infection among the medical staff in experimental group. The work efficiency (the time in completing the work per unit, 180 min vs 220 min) and satisfaction in experimental group were significantly higher than those in control group. Conclusion The list management mode on COVID-19 can effectively promote the stable and orderly operation and meet the requirements for the prevention and control of the infection in the COVID-19 designated hospital.