Abstract:Objective By detecting the expression of p53, spermidine/spermine N1-acetyltransferase 1 (SAT1) and arachidonate 15-lipoxygenase (ALOX15) proteins in the muscle tissue of experimental autoimmune myositis (EAM) mice with immunohistochemistry to explore the correlation between the pathogenesis of idiopathic inflammatory myopathy (IIM) and p53/SAT1/ALOX15 ferroptosis pathway protein. Methods Thirteen BALB/c mice were randomly divided into EAM group (n=7) and control group (n=6). EAM mouse model was established by subcutaneous injection after roughly extracted guinea pig skeletal muscle homogenate was mixed with complete Freund′s adjuvant. One week after the last immune treatment, observing the clinical manifestations of mice and measuring the weight, muscle strength of limbs, serum CK level and hematoxylineosin staining results of skeletal muscle were used to determine whether the model was successful. The expression of p53, SAT1 and ALOX15 proteins in mice muscle tissues were detected by immunohistochemistry. Results By the integrated method 6 mice in EAM group were successfully established (1 died). The positive expression rate and immunohistochemical expression score of p53, SAT1 and ALOX15 in muscle tissue of EAM model group were higher than those of control group (P<0.05, P<0.01). Pearson correlation analysis showed that p53 was positively correlated with SAT1 and ALOX15 respectively (r=0.976, P=0.001; r=0.963, P=0.002), SAT1 was positively correlated with ALOX15 (r=0.951, P=0.004). Conclusion p53/SAT1/ALOX15 ferroptosis pathway protein may be involved in the pathogenesis of idiopathic inflammatory myopathy.