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投稿时间：2020-03-15 网络发布日期：2021-02-20
投稿时间：2020-03-15 网络发布日期：2021-02-20
中文摘要: 目的 分析高迁移率族蛋白1(HMGB1)与辅助性T细胞(Th17)在阿尔兹海默症(AD)患者中的诊断价值,为临床应用提供依据。方法 选取2019年7月至2020年2月于山西医科大学第二医院就诊的107例AD患者作为AD组,选择同期的107例健康人作为对照组,用酶联免疫吸附测定法和流式细胞仪分别检测血清中的HMGB1和Th17计数,并用ROC曲线分析HMGB1及Th17单独及联合诊断AD的效能。结果 AD组HMGB1及Th17显著高于对照组,差异有统计学意义(P<0.01)。严重意识障碍AD患者血清中HMGB1及Th17显著高于轻度意识障碍患者(P<0.01)。联合检测诊断AD的特异度、敏感度高于单独HMGB1和单独Th17检测诊断。HMGB1与Th17对于AD诊断准确度的ROC曲线下面积(AUC)分别为0.754、0.812。结论 HMGB1与Th17是诊断AD的敏感度高、特异性强的标志物,有利于监测AD患者病情、观察疗效和指导治疗。二者的联合使用对于AD的鉴别诊断有重要意义。
Abstract:Objective To analyze the diagnosis value of high mobility group box 1 protein（HMGB1) combined with T helper cell 17(Th17) in Alzheimer′s disease(AD),and to provides evidence for clinical application.MethodFrom July 2019 to February 2020,107 patients from the Second Affiliated Hospital of Shanxi Medical University with AD were selected as the AD group and 107 healthy people were collected as the control group.Serum HMGB1 and Th17 levels were detected by enzyme-linked immunosorbent assay and flow cytometry.ROC curve was used to analyze the efficacy of HMGB1 and Th17 alone and in combination in diagnosing AD.Results HMGB1 and Th17 in AD group were higher than those in control group,the difference was statistically significant ( P<0.01).The serum levels of HMGB1 and Th17 in AD patients with severe consciousness disorder were higher than those in patients with mild consciousness disorder ( P<0.01).The specificity and sensitivity of combined testing in the diagnosis of AD were higher than those of HMGB1 alone and Th17 alone.The area under the ROC curve (AUC) of HMGB1 and Th17 for AD diagnosis accuracy were 0.754 and 0.812,respectively.Conclusions HMGB1 and Th17 are highly sensitive and specific markers of AD,which are helpful for monitoring the condition of AD patients,observing the efficacy and guiding treatment.The combined use of HMGB1 and Th17 is of great significance for the differential diagnosis of AD.
文章编号： 中图分类号：R749.1+6 文献标志码：B
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附件
| Author Name | Affiliation |
| HAN Rong*,HU Jin-lian | *Hong Kong Polytechnic University,Hong Kong 999077,China |
引用文本：
韩榕,胡金莲.HMGB1与Th17在阿尔兹海默症中的诊断价值[J].中国临床研究,2021,34(2):224-226.
韩榕,胡金莲.HMGB1与Th17在阿尔兹海默症中的诊断价值[J].中国临床研究,2021,34(2):224-226.