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投稿时间：2020-04-15 网络发布日期：2020-12-20
投稿时间：2020-04-15 网络发布日期：2020-12-20
中文摘要: 目的 研究姜黄素固体分散体(Cur SD)对镉染毒雄性大鼠生殖器官损伤的保护作用。方法 SPF级雄性SD大鼠40只，分为5组:空白组、模型组、Cur SD 低、中、高剂量组 。除空白组腹腔注射2 mg/kg生理盐水外，其余各组腹腔注射2 mg/kg CdCl2进行染毒，1次/2 d，共6次。同时，Cur SD 低、中、高剂量组分别给予30 mg/kg、60 mg/kg、90 mg/kg浓度的Cur SD(按姜黄素浓度计)灌胃，1次/1 d，共6周。空白组和染镉组大鼠灌胃等量1%羧甲基纤维素钠溶液，1次/d，连续6周。试验完毕，处死大鼠，摘取睾丸、附睾，计算脏器系数，精子数量，测定大鼠睾丸组织SOD、MDA、caspase-3的活力，睾丸组织镉含量，电镜观察大鼠睾丸组织病理变化。结果 镉使得大鼠脏器系数降低，精子数减少，活动率降低；给药后Cur SD高剂量提高了睾丸与附睾的脏器系数、精子数和活动率。染镉组睾丸组织中测得SOD、MDA、 caspase-3的值分别为(2.569±0.279)U/mgprot、(18.880±2.942)nmol/mgprot、(1.553±0.439)U/mgprot；Cur SD高剂量组睾丸组织中测得SOD、MDA、 caspase-3的值分别为(3.948±0.449) U/mgprot，(4.200±0.762)nmol/mgprot，(0.559±0.167)U/mgprot。染镉后睾丸与附睾组织中蓄积了较多的镉，使大鼠睾丸内SOD活力降低和MDA含量升高(P<0.05)，caspase-3含量升高(P<0.05)，给药后SOD活力升高、MDA含量降低，caspase-3含量降低，且以Cur SD高剂量组效果最好；从病理切片观察到染镉组睾丸萎缩，曲精小管破坏，生精细胞数目减少，而Cur SD高剂量组接近正常组。结论 Cur SD高剂量组使镉致雄性大鼠生殖器官的损伤减小，具有明显的保护作用，其保护作用可能与姜黄素浓度有关。
Abstract:Objective To investigate the protective effect of curcumin solid dispersion (Cur SD) on reproductive organ injury in male rats exposed to cadmium. Methods A total of 40 SPF male SD rats were selected and divided into 5 groups: blank group, cadmium exposed group, Cur SD low-dose group, Cur SD medium-dose group and Cur SD high-dose group (n=8, each).Except that the blank group was injected with normal saline (2 mg/kg, ip), the other groups were intraperitoneally injected with 2 mg/kg CdCl2, once every other day for 6 times. Cur SD low-, medium-and high-dose groups were given Cur SD of 30 mg/kg, 60 mg/kg and 90 mg/kg respectively, once a day for 6 weeks.The rats in blank group and cadmium exposed group were intragastrically administered with 1% sodium carboxymethyl cellulose solution, once a day for 6 weeks.At the end of the experiment, the rats were killed, the testis and epididymis were removed, the organ coefficient and sperm number were calculated, the activities of SOD, MDA, caspase-3, the content of cadmium in testicular tissue were measured, and the pathological changes of testicular tissue were observed by electron microscope. Results Cadmium reduced the organ coefficient, sperm number and motility rate of rats, after administration of Cur SD high dose increased the organ coefficient, sperm number and activity rate of testis and epididymis. The values of SOD, MDA and caspase-3 were (2.569±0.279) U/mgprot, (18.880±2.942) nmol/mgprot and (1.553 ±0.439) U/mgprot in testicular tissue of cadmium exposed group, and (3.948±0.449) U/mgprot, (4.200±0.762) nmol/mgprot and (0.559±0.167) U/mgprot respectively.After cadmium exposure, cadmium accumulated in testis and epididymis, which decreased SOD activity and increased MDA content (P<0.05), and increased caspase-3 content (P<0.05).After administration, SOD activity increased, MDA content decreased, caspase-3 content decreased.The results showed that the effect of high-dose SD group was the best, the testis atrophy, seminiferous tubule damage and spermatogenic cells decreased in cadmium exposed group, while the Cur SD high-dose group was close to the normal group. Conclusion Cur SD high-dose group can reduce the damage of reproductive organs induced by cadmium in male rats, and has obvious protective effect, which may be related to the concentration of curcumin.
文章编号： 中图分类号：R-33，R285.5 文献标志码：A
基金项目:河北省中医药管理局项目(2016194)
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引用文本：
张钰欣，李琳玉，张臻臻，赵利刚，董红红，赵琳琳.姜黄素固体分散体对镉致雄性大鼠生殖器官损伤的保护作用[J].中国临床研究,2020,33(12):1607-1610,1615.
张钰欣，李琳玉，张臻臻，赵利刚，董红红，赵琳琳.姜黄素固体分散体对镉致雄性大鼠生殖器官损伤的保护作用[J].中国临床研究,2020,33(12):1607-1610,1615.