Abstract:ObjectiveTo explore the relationship between adenosine deaminase(ADA) gene polymorphism and recurrent spontaneous abortion(RSA).MethodsA total of 300 patients admitted to Beijing Jishuitan Hospital from January 2016 to January 2018 were randomly selected,including 120 RSA patients as experimental group and 180 non-RSA patients as control group.After collecting the patients′s venous blood,ADA G22A gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).ResultsThere were no significant differences in genotype and allele frequencies of ADA between two groups (P>0.05);and also no significant differences found in frequency distribution of ADA genotype of pregnant women complicated with diabetes mellitus,hypertension,polycystic ovary syndrome,uterine malformation,positive anti-phospholipid antibody and endometriosis in experimental group (P>0.05).There was no significant difference in genotype and allele frequency distribution of ADA in the patients aged less than 35 years between two groups (P>0.05),but statistical differences were obsewed in them for the patients aged more than 35 years between two groups(P=0.005).Compared with GA genotype,GG genotype significantly increased the risk for RSA (OR=0.113,95%CI=0.022-0.585).There was a statistical difference in allele frequency distribution between two groups(P=0.001).Compared with A allele,G allele showed a statistically increased risk for RSA(OR=9.774,95 %CI=2.051-46.574).ConclusionsThe polymorphism of ADA gene is closely related to the occurrence of RSA in the pregnant women over 35 years old,which has a certain predictive value for the possibility of abortion in older pregnant women.