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投稿时间：2019-12-11 网络发布日期：2020-06-20
投稿时间：2019-12-11 网络发布日期：2020-06-20
中文摘要: 目的 研究淋巴增强因子1(LEF1)的表达与食管鳞癌放疗敏感性的关系并进行机制探讨。方法 选择2010年至2016年期间在唐山市人民医院进行放射治疗的食管鳞癌患者59例,均为不能接受手术,经穿刺诊断为局部晚期食管鳞癌的患者,均接受根治性放疗。采用免疫组织化学法检测上述食管癌病理组织中LEF1蛋白的表达。分析LEF1表达情况与患者放疗疗效及患者年龄、有无淋巴结转移、临床分期等临床病理特征之间的关系。结果 所有患者放射治疗均顺利完成,无明显不良反应。59例食管癌患者放疗有效为(CR 13例、PR 17例)30例,放疗无效为(NR)29例。放疗有效组30例核表达阳性9例,阳性表达率30.0%,无效组核表达阳性18例,阳性表达率62.1%,相对于有效组,LEF1蛋白核表达水平在无效组中高表达,差异具有统计学意义(χ2=6.110,P=0.013)。相对于无淋巴结转移组,LEF1蛋白表达水平在有淋巴结转移组肿瘤组织中高表达,两组差异有统计学意义(χ2=4.489,P=0.034);Ⅲ～Ⅳ期病例肿瘤组织中的LEF1表达高于Ⅰ～Ⅱ期病例,两组差异有统计学意义(χ2=5.879,P=0.015);LEF1在不同年龄、性别、不同长度的肿瘤组织中的表达无显著差异(P>0.05)。结论 食管癌组织中LEF1表达程度可能是预测食管鳞癌放射抗性分子指标。LEF1与食管鳞癌的淋巴结转移及临床分期相关,可能参与食管鳞癌侵袭、转移的病理过程。
Abstract:Objecitve To study the relationship between the expression of lymphoid enhancer factor-1(LEF1) and the radiosensitivity of esophageal squamous cell carcinoma(ESCC) and its mechanism. Methods Fifty-nine ESCC patients who received radical radiotherapy instead of operation because of locally advanced ESCC confirmed by needle puncture from 2010 to 2016 were selected.The expression level of LEF1 protein in esophageal cancer tissue was detected by immunohistochemistry.The associations of LEF1 expression with the therapeutic effect of radiotherapy, age, lymph node metastasis, clinical stage and other clinicopathological characteristics was analyzed. Results The radiotherapy was successfully completed in all patients without obvious adverse reactions.There were 13 cases of complete remission(CR), 17 cases of partial remission(PR) and 29 cases of non-remission (NR). The nuclear expressions of LEF1 were positive in 9 of 30 patients with effective radiotherapy(CR and PR group, 30.0%) and in 18 of 29 patients with ineffective radiotherapy (NR group, 62.1%).There was a statistical difference between two groups (χ2=6.110, P=0.013).LEF1 expression levels were significantly higher in the patients with lymph node metastasis than those of the patients without lymph node metastasis(χ2=4.489，P=0.034) and also in the patients with stage III-IV ESCC than those of the patients with stage Ⅰ-Ⅱ ESCC (χ2=5.879，P=0.015).LEF1 expression levels were significantly higher in the patients with lymph node metastasis than those in the patients without lymph node metastasis(χ2=4.489，P=0.034) and also in the patients with stage III-IV ESCC than those in the patients with stage Ⅰ-Ⅱ ESCC (χ2=5.879，P=0.015).There were no statistical differences in LEF1 expression levels of the patients with different ages, genders and tumor size(P>0.05). Conclusion LEF1 expression level may be a molecular marker for predicting radiation resistance of esophageal squamous cell carcinoma.LEF1 is related to lymph node metastasis and clinical stages of ESCC and may be involved in the pathological process of invasion and metastasis of ESCC.
keywords: Lymphoid enhancer factor-1 Esophageal squamous cell carcinoma Radiotherapy Lymph node metastasis Clinical stage
文章编号： 中图分类号： 文献标志码：B
基金项目:唐山市科技局计划项目(17130238a)
附件
| Author Name | Affiliation |
| HUANG Xiao-zhi, LIU Yang, YI Xuan-hong, XIONG Wei | Department of Radiochemistry, Tangshan People′s Hospital, Tangshan, Hebei 063000, China |
引用文本：
黄晓智,刘洋,易宣洪,熊伟.淋巴增强因子1的表达与食管鳞癌放疗敏感性的关系[J].中国临床研究,2020,33(6):799-802.
黄晓智,刘洋,易宣洪,熊伟.淋巴增强因子1的表达与食管鳞癌放疗敏感性的关系[J].中国临床研究,2020,33(6):799-802.