Abstract:Objective To investigate the expression and clinical significance of transcription factor GATA6 and transient receptor potential cation channel protein(TRPC)6 in colon cancer tissues. Methods A total of 136 patients with colon cancer confirmed by pathology in the Fourth Central Hospital of Baoding and the Second Hospital of Baoding were selected.Immunohistochemistry and enzyme-linked immunosorbent assay(ELISA)were used to detect the expression levels of GATA6 and TRPC6 in colon cancer tissues (colon cancer group) and normal colon tissues(para-cancerous group) to analyze the associations of the expressions of GATA6 and TRPC6 with the clinicopathological characteristics and prognosis of patients with colon cancer. Results The immunohistochemical results showed that 102 cases were positive for GATA6,and 106 cases were positive for TRPC6 in colon cancer group;the positive rates of GATA6（75.00% vs 13.24%,P<0.01）and TRPC6（77.94% vs 4.41%,P<0.01）in colon cancer group were significantly higher than those in para-cancerous group.The expression scores of GATA6 and TRPC6 in colon cancer group were statistically higher than those in para-cancerous group (all P<0.01).The expressions of GATA6 and TRPC6 protein were not significantly correlated with age (all P>0.05),but were significantly correlated with the largest diameter of tumor,pathological grade,TNM stage,infiltration depth,lympha-vascular space invasion,lymph node metastasis,and recurrence (all P<0.01).The 5-year survival rates in GATA6 negative group (76.47% vs 29.41%) and in TRPC6 negative group (80.00% vs 30.19%) were significantly higher than those in GATA6 and TRPC6 positive groups respectively,and the survival periods were also significantly higher than those in GATA6 and TRPC6 positive groups (all P<0.01). Conclusions The high expression levels of GATA6 and TRPC6 in colon cancer tissues may play a promoting role in the development of colon cancer.The patients with low expressions of GATA6 and TRPC6 may obtain a good prognosis.