Abstract:Objective To investigate the expression of extracellular signalregulated kinase (ERK),vascular endothelial growth factor (VEGF) and matrix metalloproteinase9 (MMP9) in 4nitroquinoline 1oxide (4NQO)induced mouse esophageal cancer cells.Methods A total of 200 female BABL/c mice were randomly divided into 4 groups (normal group,model 10week group,model 15week group and model 20week group;n=50,each).The 4NQO was used to construct esophageal cancer model in the three model groups.Body weight changes were monitored during the period,and all of them were anesthetized and executed 20 weeks later.The cancerization rate was observed and esophageal cancer cells were taken in the three model groups and esophageal cancer cells were taken from normal group.MTT method was used to detect cell viability,RTPCR and Western blot were used to detect mRNA and protein expression.Results On the 2nd day,10th,15th and 20th week,there was no significant difference in the body weight of mice in normal group (P>0.05),and the weight of mice in three model groups decreased significantly with the time of modeling (P<0.01).MTT results showed that after 20 weeks of feeding,the proliferation rate of esophageal cancer cells in the model 10week group,model 15week group and model 20week group was significantly higher than that normal cells(P<0.01);with the extension of modeling time (10 weeks → 15 weeks → 20 weeks),the proliferation rate of esophageal cancer cells of each model groups increased significantly(P<0.01).The results of RTPCR showed that expression of ERK、VEGF and MMP9 mRNA in cancer cells of each model group increased gradually with the prolongation of modeling time (10 weeks→15 weeks→20 weeks)(P<0.01).Western blot showed that the expression of ERK,VEGF and MMP9 protein increased gradually with the prolongation of modeling time(10 weeks→15 weeks→20 weeks)(P<0.01).Conclusion ERK、VEGF、MMP9 may be directly related to the proliferation and prognosis of esophageal cancer cells.