Abstract:ObjectiveTo analyze the value of bone marrow smear combined with immunotyping in early diagnosis of bone marrow involvement in lymphoma cell leukemia (LMCL). Methods The clinical data of 80 patients with LMCL were studied retrospectively.The morphological characteristics of bone marrow smear and the bone marrow immunotyping were observed to analyze the value of bone marrow smear combined with immunotyping in the early diagnosis of LMCL bone marrow involvement furtherly. Results Typical lymphoma cells were found in bone marrow of 80 patients.The differentiation antigen expression in immunophenotyping was consistent with the pathological type of the original disease,including NK/T cell type in 7 cases (8.75%),B lymphocyte type in 44 cases (55.00%) and T lymphocyte type in 29 cases (36.25%).In fewer cases of NK/T cell type,there were the expressions of CD7,CD56,CD38,CD161,CD94 and CD15.In B-cell type LMCL patients,there were the expressions of Skappa,CD79a,CD22,CD19,CD20 and early antigen HLA-DR,with the highest positive rate of CD19 (88.94%).CD2,CD3,CD11b,CD7,CD5,CD34 and HLA-DR were mainly expressed in T-cell type LMCL patients,in which CD7 was the most sensitive,with the positive rate of 68.97%.The proportion of lymphoma cells in bone marrow smear was consistent with that in immunotyping in 65 cases (81.25%). ConclusionIn early diagnosis of LMCL bone marrow involvement.Both bone marrow smear and immunotyping have certain value and can complement each other for the early diagnosis of LMCL.