Abstract:Objective To investigate the value of endometrium thickness detected by trans-vaginal ultrasound combined with serum β-human chorionic gonadotropin (HCG) and carbohydrate antigen (CA)-125 detection in the diagnosis of ectopic pregnancy.? Methods From January to June 2017, 22 patients with ectopic pregnancy (ectopic group) and 25 normal pregnant women (normal group) were selected as study object. The level of serum β-HCG and CA125 and endometrium thickness were detected and compared in two groups. The diagnosis efficiency of ectopic pregnancy was compared between the 3 indexes respectively and generally.? Results Compared to normal group, the serum β-HCG, CA125 progesterone level and endometrium thickness were all lower than those in ectopic group(all P<0.01). After 48 hours, the rate of doubling β-HCG in ectopic group was significant lower than that in normal group［13.6%(3/22) vs 96.0%(24/25), χ²=32.474, P<0.01] . The area under the curve (AUC) of serum β-HCG, CA125 and endometrial thickness for the diagnosis of ectopic pregnancy was 0.689(95%CI:0.536 to 0.842), 0.998(95%CI:0.992 to 1.000) and 0.996(95%CI:0.987 to 1.000), respectively. The AUC of combined diagnostic was 1.? Conclusion Detecting the serum levels of β-HCG and CA125, combined with endometrium thickness detected by trans-vaginal ultrasound, could improve the accuracy of diagnosis of ectopic pregnancy significantly.